Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study; data were taken from a structural similar substance (CAS 81-33-4)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Lot/batch No.: Partie 18
- Molecular formula: C24 H10 N2 O4
- Molecular weight (g/mol): 390.36
- Aggregate state: solid
- Water-solubility: <500 mg/L

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage.
- Concentration of sludge: 30 mg/I
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature:
- pH: 7.2 - 7.5

TEST SYSTEM
- Number of culture flasks/concentration: 7

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
0 - 10
Sampling time:
28 d
Details on results:
- Degradation degree of the test substance after 28 days (% BOD/ThOD) : 0-10
- Degradation degree of the test substance at the end of the test (% BOD/ThOD) : 0-10

BOD5 / COD results

Results with reference substance:
- Degradation degree of the reference substance after 14 days (% BOD/ThOD) : 70-80

Applicant's summary and conclusion

Interpretation of results:
other: poorly biodegradable