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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Scientifically acceptable publication; although the exposure period was reduced to 24 h, sufficient data was given in the publication on testing procedure to justify the study as val2 for the endpoint short-term toxicity to aquatic invertebrates.

Data source

Reference
Reference Type:
publication
Title:
Biological effects of prenylated hydroquinones: structure-activity relationship studies in antimicrobial, brine shrimp, and fish lethality assays
Author:
De Rosa, S., De Giulio, A. and Iodice, C.
Year:
1994
Bibliographic source:
Journal of Natural Products, Vol. 57, No. 12, pp. 1711-1716

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
A test guideline was not mentioned in the publication.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylhydroquinone
EC Number:
202-443-7
EC Name:
2-methylhydroquinone
Cas Number:
95-71-6
Molecular formula:
C7H8O2
IUPAC Name:
2-methylbenzene-1,4-diol
Constituent 2
Reference substance name:
Methylhydroquinone
IUPAC Name:
Methylhydroquinone
Details on test material:
- Origin of test material: Sigma-Aldrich s.r.l., Italy

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION OF TEST SOLUTION
- Test item was dissolved in DMSO (1% final volume)

Test organisms

Test organisms (species):
Artemia salina
Details on test organisms:
- Organism: Brine Shrimp (freshly hatched larvae)

Study design

Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
24 h

Test conditions

Nominal and measured concentrations:
- Concentrations tested (nominal): 10, 0.1 and 0.01 ppm
Details on test conditions:
TEST SYSTEM
- No. of replicates/concentration: 3
- No. of organisms/replicate: 10
- Introduction of organism to test medium: freshly hatched larvae were placed in 5 mL artificial sea water to which appropriate amounts of compound (dissolved in DMSO) were added.

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
> 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Reported statistics and error estimates:
The data was statistically analyzed by the Finney program.

Applicant's summary and conclusion