Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 215-174-5 | CAS number: 1309-60-0
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- other: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No GLP, documentation is not guideline compliant, e.g. data for positive control substance are missing thus raising concerns on the validity of the study
- Justification for type of information:
- Read-across from supporting substance (structural analogue or surrogate), same metal ion than the substance of interest.
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity of heavy metals
- Author:
- Wong, P.K.
- Year:
- 1 988
- Bibliographic source:
- Bulletin of Environmental Contamination & Toxicology 1988, 40, (4), 597-603
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- no positive control substances, number of replicates is not reported, no data for TA 100 , raw data not reported (no revertant numbers), evaluation criteria not reported
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- sterilized solution of lead chloride in 0.1 M citric acid
- IUPAC Name:
- sterilized solution of lead chloride in 0.1 M citric acid
- Test material form:
- liquid
Constituent 1
Method
- Target gene:
- HIS operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535
- Species / strain / cell type:
- S. typhimurium TA 1537
- Species / strain / cell type:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 20 ppm
- Vehicle / solvent:
- 0.1 M citric acid
- Details on test system and experimental conditions:
- plate-incorporation method
- Evaluation criteria:
- no details
- Statistics:
- no details
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field "Test system"
Applicant's summary and conclusion
- Conclusions:
- positive without metabolic activation in TA102
- Executive summary:
It is concluded that the test substance is mutagenic in TA102 without metabolic activation in this test system.
It is reasonable to assume that the mutagenic property of lead salts is triggered by the lead cation and not the corresponding anion.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
