C14-16 (even numbered) and C16 (branched) saturated and unsaturated aliphatic hydrocarbons

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, non guideline, available as unpublished report, acceptable with restrictions

Justification for type of information:

A discussion and report on the read across strategy is given as an attachment in Section 13.

Cross-reference

Reason / purpose for cross-reference:

read-across: supporting information

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, non guideline, available as unpublished report, acceptable with restrictions

Justification for type of information:

A discussion and report on the read across strategy is given as an attachment in Section 13.

Reason / purpose for cross-reference:

read-across source

Principles of method if other than guideline:

The acute and chronic toxicity of the test substance to earthworms was tested in a laboratory bioassay. Natural soil exposed in the field to the test substance was sampled over time. The bulk sample was analysed for physico chemical parameters and the concentration of the test substance. Earthworms were exposed to the soil samples and either their 14 day survival or 70 day effects on growth and reproducion recorded.

GLP compliance:

no

Remarks:

no data reported

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data reported

Analytical monitoring:

yes

Details on sampling:

A natural silty loam soil was dosed with drill cuttings containing the test substance in September 2001. Soil samples were taken after 2.5, 7, 8 and 10.5 months. 15 soil samples from 0-15cm depth were taken on each occassion, spread evenly across the test and control plots. The samples were bulked and homogenised.

Vehicle:

no

Details on preparation and application of test substrate:

Soil samples collected in the field determined the test concentrations. The bulk soil samples had measured corrected concentrations of 31428, 25195, 8145 and 10026ug/g dry weight over time. Only the concentration of 10026ug/g was tested for chronic effects on earthworms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

large, healthy, adult worms were used

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

yes

Total exposure duration:

70 d

Post exposure observation period:

none

Test temperature:

22±2°C

pH:

6.5-7.7

Moisture:

remoistened

Details on test conditions:

three replicates of 500cc of remoistened soil were weighed out and placed in 900ml glass jarrs. The earthworms were added to the soil surface and the jars closed with a vented lid. The test was conducted under continual illumination.

Nominal and measured concentrations:

control and 10026ug/g dry weight (measured)

Reference substance (positive control):

no

Duration:

70 d

Dose descriptor:

NOEC

Effect conc.:

10 026 other: µg/g dry weight

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

70 d

Dose descriptor:

EC100

Effect conc.:

10 026 other: µg/g dry weight

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

70 d

Dose descriptor:

EC50

Effect conc.:

10 026 other: µg/g dry weight

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other: approximate

Details on results:

No effects on mortality were seen at any test concentration

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Not applicable

Validity criteria fulfilled:

not specified

Remarks:

non standard test

Conclusions:

At the test concentration no effects on mortality were observed but reproduction was completely inhibited.

Executive summary:

At the test concentration no effects on mortality were observed but reproduction was completely inhibited. Although this test does not follow standard guidelines it is well described and includes analytical monitoring of the exposure concentrations. No effects on mortality were observed at the test concentration, however reproduction was completely inhibited. The NOEC reproduction cannot be calculated from this test, and this should be borne in mind whilst deriving the soil PNEC.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Principles of method if other than guideline:

The acute and chronic toxicity of the test substance to earthworms was tested in a laboratory bioassay. Natural soil exposed in the field to the test substance was sampled over time. The bulk sample was analysed for physico chemical parameters and the concentration of the test substance. Earthworms were exposed to the soil samples and either their 14 day survival or 70 day effects on growth and reproducion recorded.

GLP compliance:

no

Remarks:

no data reported

Test material

Test material information

Constituent 1

Reference substance name:

1-Tetradecene

IUPAC Name:

1-Tetradecene

Constituent 2

Reference substance name:

Tetradec-1-ene

EC Number:

214-306-9

EC Name:

Tetradec-1-ene

Cas Number:

1120-36-1

Molecular formula:

C14H28

IUPAC Name:

tetradec-1-ene

Details on test material:

Amodrill 1410, linear alpha olefin C14

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data reported

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

A natural silty loam soil was dosed with drill cuttings containing the test substance in September 2001. Soil samples were taken after 2.5, 7, 8 and 10.5 months. 15 soil samples from 0-15cm depth were taken on each occassion, spread evenly across the test and control plots. The samples were bulked and homogenised.

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

Soil samples collected in the field determined the test concentrations. The bulk soil samples had measured corrected concentrations of 31428, 25195, 8145 and 10026ug/g dry weight over time. Only the concentration of 10026ug/g was tested for chronic effects on earthworms

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

large, healthy, adult worms were used

Study design

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

yes

Total exposure duration:

70 d

Post exposure observation period:

none

Test conditions

Test temperature:

22±2°C

pH:

6.5-7.7

Moisture:

remoistened

Details on test conditions:

three replicates of 500cc of remoistened soil were weighed out and placed in 900ml glass jarrs. The earthworms were added to the soil surface and the jars closed with a vented lid. The test was conducted under continual illumination.

Nominal and measured concentrations:

control and 10026ug/g dry weight (measured)

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

70 d

Dose descriptor:

NOEC

Effect conc.:

10 026 other: µg/g dry weight

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

70 d

Dose descriptor:

EC100

Effect conc.:

10 026 other: µg/g dry weight

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 3

Duration:

70 d

Dose descriptor:

EC50

Effect conc.:

10 026 other: µg/g dry weight

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other: approximate

Details on results:

No effects on mortality were seen at any test concentration

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Not applicable

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Remarks:

non standard test

Conclusions:

At the test concentration no effects on mortality were observed but reproduction was completely inhibited.

Executive summary:

At the test concentration no effects on mortality were observed but reproduction was completely inhibited. Although this test does not follow standard guidelines it is well described and includes analytical monitoring of the exposure concentrations. No effects on mortality were observed at the test concentration, however reproduction was completely inhibited. The NOEC reproduction cannot be calculated from this test, and this should be borne in mind whilst deriving the soil PNEC.