C14-16 (even numbered) and C16 (branched) saturated and unsaturated aliphatic hydrocarbons

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March to November 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: This robust summary has a reliability rating of 1 because the study followed a standard guideline, followed GLP guidelines, and was conducted without deviations that would invalidate the study.

Justification for type of information:

A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.

Cross-reference

Reason / purpose for cross-reference:

read-across: supporting information

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

March to November 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: This robust summary has a reliability rating of 1 because the study followed a standard guideline, followed GLP guidelines, and was conducted without deviations that would invalidate the study.

Justification for type of information:

A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

An equilibration study was conducted prior to the study to determine the length of time needed to develop water accommodated fractions (WAFs) at the 1000 mg/L loading level. The aqueous phase of the WAF was analyzed after 1, 24, 48, and 96 hours of stirring. In all samples, the quantity of test substance in solution was less than the limit of detection (0.5 mg/L). Further analysis of test substance in test media a loading level of 1 g/L also showed no detectable amounts of test substance. Consequently, analysis of treatment solutions from the definitive study were not conducted.

Vehicle:

no

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Organisms used in the test were from a laboratory culture, originally derived from a strain (ATCC 22662) obtained from the American Type Culture Collection, Maryland, Ohio, USA.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

None

Test temperature:

23 to 25 degrees C

pH:

7.2 to 8.8

Nominal and measured concentrations:

The nominal loading rate was 1000 mg/L. A control was also tested.

Details on test conditions:

The study was conducted in sealed test systems with no headspace that were not renewed during the study. The test systems used were 300 ml glass Erlenmeyer flasks that were complely filled with test media. Six replicate test systems were established for the treatment level and control. Control and treatment systems were innoculated with a concentration of 5000 cells/ml. Test systems were incubated under constant illumination (approximately 3000 lux) on an orbital incubator.

Reference substance (positive control):

no

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

NOELR

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Duration:

72 h

Dose descriptor:

NOELR

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Validity criteria fulfilled:

yes

Conclusions:

Growth of an alga culture, Pseudokirchnerella subcapitata, as measured by biomass and growth rate, exposed to a 1000 mg/L water accommodated fraction of the test substance was not inhibited when compared to the control after a 72-hour exposure. Therefore, the 72-hr EL50 and NOELR values for these two endpoints are reported as >1000 mg/L and 1000 mg/L, respectively.

Executive summary:

Growth of an alga culture, Pseudokirchnerella subcapitata, as measured by biomass and growth rate, exposed to a 1000 mg/L water accommodated fraction of the test substance was not inhibited when compared to the control after a 72-hour exposure. Therefore, the 72-hr EL50 and NOELR values for these two endpoints are reported as >1000 mg/L and 1000 mg/L, respectively.

Data source

Reference

Reference Type:

other: Study report; company data

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Hydrocarbons, C11-C14, n-alkanes, isoalkanes, cyclics, <2% aromatics

EC Number:

926-141-6

IUPAC Name:

Hydrocarbons, C11-C14, n-alkanes, isoalkanes, cyclics, <2% aromatics

Details on test material:

Hydrocarbons, C11-C14, containing n-alkanes, isoalkanes, cyclics with <2% aromatics

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

An equilibration study was conducted prior to the study to determine the length of time needed to develop water accommodated fractions (WAFs) at the 1000 mg/L loading level. The aqueous phase of the WAF was analyzed after 1, 24, 48, and 96 hours of stirring. In all samples, the quantity of test substance in solution was less than the limit of detection (0.5 mg/L). Further analysis of test substance in test media a loading level of 1 g/L also showed no detectable amounts of test substance. Consequently, analysis of treatment solutions from the definitive study were not conducted.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Organisms used in the test were from a laboratory culture, originally derived from a strain (ATCC 22662) obtained from the American Type Culture Collection, Maryland, Ohio, USA.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

None

Test conditions

Test temperature:

23 to 25 degrees C

pH:

7.2 to 8.8

Nominal and measured concentrations:

The nominal loading rate was 1000 mg/L. A control was also tested.

Details on test conditions:

The study was conducted in sealed test systems with no headspace that were not renewed during the study. The test systems used were 300 ml glass Erlenmeyer flasks that were complely filled with test media. Six replicate test systems were established for the treatment level and control. Control and treatment systems were innoculated with a concentration of 5000 cells/ml. Test systems were incubated under constant illumination (approximately 3000 lux) on an orbital incubator.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOELR

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOELR

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Growth of an alga culture, Pseudokirchnerella subcapitata, as measured by biomass and growth rate, exposed to a 1000 mg/L water accommodated fraction of the test substance was not inhibited when compared to the control after a 72-hour exposure. Therefore, the 72-hr EL50 and NOELR values for these two endpoints are reported as >1000 mg/L and 1000 mg/L, respectively.

Executive summary:

Growth of an alga culture, Pseudokirchnerella subcapitata, as measured by biomass and growth rate, exposed to a 1000 mg/L water accommodated fraction of the test substance was not inhibited when compared to the control after a 72-hour exposure. Therefore, the 72-hr EL50 and NOELR values for these two endpoints are reported as >1000 mg/L and 1000 mg/L, respectively.