Alcohols, C12-18, reaction products with D-glucose

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: There were no circumstances that may have affected the quality or integrity of the study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

2007-01-12

Test material

Test material information

Constituent 1

Reference substance name:

Montanov 82

IUPAC Name:

Montanov 82

Details on test material:

- Batch n°: T62935
- Appearence: pearls of wax
- Purity: ES>99%
- Stability: water 96 h
- Solubility: water<0.1g/L
- Production date 2006/07/20
- Expiry date 2008/01/19
- Storage: room temperature 20 +/-5°C

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

As solubility lies below 100mg/L, the water-accomodated fraction was prepared for the test. This was done by weighing the nominal load, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45um filters.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Variety: STRAUSS
- Strain: Berlin
- Sex: female
- Age: between 0 and 24 hours
- Origin: Umweltbundesamt Berlin
- Arrival of Strain: October 4th 2005
- Food: unicellular green algae (Desmodesmus subspicatus)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

21.9-22.5°C

pH:

7.7-7.9

Dissolved oxygen:

8.3mg/L

Nominal and measured concentrations:

0 / 1 / 10 / 100 mg/L

Details on test conditions:

The water accomodated fraction was prepared by weighing of the nominal load, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45 um filters.
Vessels: glass beakers

-Main study and reference study:
Replicates: four vessels, each containing 20 mL test solution and five daphnia
Control: four vessels, each containing 20 mL dilution water and five daphnia
Mediumrenewal: twice a week
Photo period: 16/8 hours, using neon tubes

Reference substance (positive control):

yes

Remarks:

K2Cr2O7

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

immobilisation in the control was 10% at the most
the lowest concentration of dissolved oxygen at the end of the test was 8.3 mg/L
the highest variation of the pH value in the test solutions was 0.2 units

Results with reference substance (positive control):

24h-EC50i was determined as 1.4 mg/L (lying within the range demanded of 0.6 - 2.1 mg/L)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Duration Endpoint Effect conc.
24 h NOEC ca. 100 mg/L
24 h EC50 > 100 mg/L
24 h EC100 > 100 mg/L
48 h NOEC ca. 100 mg/L
48 h EC50 > 100 mg/L
48 h EC100 > 100 mg/L

Executive summary:

This study was performed in order to evaluate the toxic potential of Montanov 82 towards freshwater shrimp, using the species Daphnia magna. D. magna STRAUSS, which belongs to the family of crustacea, was chosen in the guideline as a typical part of zoo-plancton.

This study was conduced in accordance of the following guidelines:

OECD guideline for testing of chemicals nµ° 202, adopted April 13th 2004.

EU-guideline C.2. "acute toxicity for Daphnia" adopted July 31st 1992.

This study was conduced in compliance with the GLP.

As solubility lies below 100mg/L, the water-accomodated fraction was prepared for the test. This was done by weighing the nominal load, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45um filters.

In the main study, three concentrations of the test item in a geometric series (spaced by factor 10, the highest being 100mg/L) were used. Twenty Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.

No significant immobilisation was observed in the treatments and in the control.

The 24h-EC50i of potassium dichromate was tested in a current reference test. The value was determined as 1.4 mg/L, lying within the demanded range of 0.6 -2.1 mg/L.

The following results could be determined for the test item Montanov 82 (species: Daphnia magna)

24 h NOEC = 100 mg/L

24 h EC50i > 100 mg/L

48 h NOEC = 100 mg/L

48 h EC50i > 100 mg/L