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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The 96 h LC50 value was determined at a nominal concentration of 0.33 mg/l. This corresponds to an actual concentration of 0.21 mg/l.
The corrected actual NOEC is calculated at 0.065 mg/l.

Key value for chemical safety assessment

LC50 for freshwater fish:
0.21 mg/L

Additional information

The acute fish toxicity of Hydroxyambran was determined according to the OECD Test Guideline 203 using Rainbow trout (Oncorhynchus mykiss) as test fish.

During the preliminary test, a mortality of 100 % was observed at the highest concentration level (saturated solution, approxm. nominal 1 g/l), whereas a mortality of 20 % was observed at a dilution of 1:10, within 96 h.

The main test was performed under semi-static conditions (daily renewal of the test solutions) with 6 concentration levels of the test article based on nominal values between 1.030 mg/l and 0.058 mg/l. The serial dilution factor was 1.78.

The LC50 values were calculated as geometric mean from the LC0 and LC100. The 96 h LC50 value was determined at a nominal concentration of 0.33 mg/l. This corresponds to an actual concentration of 0.21 mg/l.

On the basis of the observations performed during the main test, the NOEC was determined at a nominal concentration of 0.103 mg/l. The corrected actual NOEC is calculated at 0.065 mg/l.