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EC number: 207-223-4 | CAS number: 454-31-9
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3120 (Sealed Vessel Carbon Dioxide Production Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Ethyl difluoroacetate
- EC Number:
- 207-223-4
- EC Name:
- Ethyl difluoroacetate
- Cas Number:
- 454-31-9
- Molecular formula:
- C4H6F2O2
- IUPAC Name:
- ethyl difluoroacetate
- Details on test material:
- - Name of test material (as cited in study report): Ethyl difluoroacetate
- Physical state: liquid / colorless, clear
- Analytical purity: 99.9%
- Batch No.: 33244/106 A
- Storage condition of test material: Storage at refrigerator, avoid temperatures >70 °C
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: municipal activated sludge from the wastewater treatment plant of the city Mannheim, Germany
- Preparation of inoculum for exposure: A suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh with a mesh size about 1 mm. After settling the supernatant was discarded and the sludge suspension was filled up with tap water. This suspension was pre-aerated for about 24 hours at room temperature. The sludge of the suspension was adjusted to a concentration 800 mg/L of dry weight and then added to the
test vessels to obtain a sludge concentration of 4 mg/L dry substance.
- Concentration of sludge: 4 mg/L dry substance - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 52 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- TOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 160 ml (aqueous phase) containing 4.4 µl (52.0 mg/L) test substance, 90 ml mineral medium, 0.5 ml (800 mg/L dry matter) inoculum, 9.5 ml water
- Test temperature: 22 ± 2 °C
- pH: 7.4 ± 0.2
- pH adjusted: no
TEST SYSTEM
- Culturing apparatus: 160 ml incubation bottles filled up to a volume of 100 ml closed with butyl rubber septa gas tight
and shaken
- Number of culture flask
- 23 blank control assays
- 23 test substance assays
- 23 inhibition control test assays
- 14 reference substance assays
- 5 assays for abiotic control
SAMPLING
- Sampling frequency: at Day 3, 7, 10, 14, 21, 28
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (TOC removal)
- Value:
- 40 - 50
- Sampling time:
- 28 d
- Details on results:
- - Duration of the adaptation phase: 2 days
- Elimination of the test substance at physical chemical control at the end of exposure (%TIC/TOC): < 10
- Degradation degree in the inhibition control after 14 days (% TIC/TOC): 48
BOD5 / COD results
- Results with reference substance:
- - Degradation degree of the reference substance after 14 days (% TIC/TOC): 60%
Applicant's summary and conclusion
- Interpretation of results:
- other: partly/moderately biodegradable
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