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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Feb 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to OECD guideline under GLP
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Principles of method if other than guideline:
not relevant
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,2 Methylen-4,6-Dien
- Analytical purity: 87,5%
- Lot/batch No.: 608/2

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 6 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Table 1: Cumulative immobilization (%) of Daphnia magna (n=20) after exposure to 1,2 Methylen-4,6 -Dien

 Dilution    Concentration of ZK 12126    24 hours    48 hours    
 Control    0,0 mg/I   0 5  
 1:100    0,1 mg/I 2)   0 0  
 1:10    0,6 mg/I 2)   0 0  
 Saturated Solution    6,0 mg/I 1)   10 10  

1) mean of two time-points, 2) extrapolated from highest concentration

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50/48 hours of 1,2 Methylen-4,6-Dien to Daphnia magna is higher than a saturated solution
(equivalent to 6.0 mg/L measured by TOC analysis). Accordingly, ZK 12126 is not toxic to Daphnia magna.
Executive summary:

The purpose of this study was to determine the acute toxicity (immobilization, EC50/48 hours) of 1,2 Methylen-4,6 -Dien to Daphnia magna. The study was conducted in agreement with the test guideline of the OECD, no. 202, "Daphnia spec., acute immobilization test"; adopted: 04 Apr. 84.

20 juvenile Daphnia magna were used for each test solution and 20 for the tap water control. The Daphnia were exposed for a period of 48 hours under static conditions. Immobilization was recorded at 24 hours and 48 hours. For the preparation of the test solution, a suspension of 100 mg/L was stirred for 24 hours. This suspension was filtered through a glassfibre filter. The resulting solution served as the highest concentration (saturated solution). It was further diluted 1:10 and 1:100.

The organic carbon concentration of the test solutions was analysed with a TOC analyser at the start of exposure and 48 hours thereafter. The analytical results gave a concentration of 6.0 mg/L (saturated solution) based on a calculation using the structural formula of the test substance. The dilutions had concentrations below the detection limit (1 mg/L organic carbon).

The EC50/48 hours of 1,2 Methylen,4,6 -Dien to Daphnia magna is higher than a saturated solution (equivalent to 6.0 mg/L measured by TOC analysis). Accordingly, the test substance is not toxic to Daphnia magna.