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EC number: 608-209-4 | CAS number: 284461-73-0
Fish larvae hatched 4 days after the introduction of the eggs in all test solutions. The hatching success was > 66 % in all test and control solutions. The number of dead fish increased with increasing concentration. From the second highest concentration on, fish growth (weight and length) decreased. The NOEC and LOEC for weight and length was 2.4 ¿g/L and 14.8 ¿g/L, respectively, for survival the NOEC was < 1.0 ¿g/L and the LOEC was ¿ 1.0 ¿g/L and for hatchability NOEC and LOEC were ¿14.8 ¿g/L.
In order to define a threshold for toxic effects, the EC10 for weight and length inhibition was determined with 0.47 ¿g/L and 0.32 ¿g/L, respectively, for survival (28 dph) was 0.17 ¿g/L.
The eggs and larvae were exposed to three test solutions of sorafenibtosylate with nominal
concentrations of 6, 17 and 50 ¿g (nominal) per liter tap water and additionally to a vehicle
(dimethylformamide, DMF) and a control for a period of 28 days post hatch (ph). For the
preparation of the stock solution, the test substance was dissolved in DMF.
The stock solution was mixed with tap water and delivered continuously to the tanks.
Mortalities and visible abnormalities were recorded daily over the exposure phase. Hatching
success and survival were recorded during the first phase of embryonic development as well as
growth and survival during the larval stage until the end of the exposure period. The
hydrographic parameters were recorded weekly.
The oxygen concentration in the test vessels was >60 % of air saturation value, the
temperature was maintained at 24 ± 2 ºC and the pH-value between 6.5 and 8.5.
Samples for the concentration analysis in the test solutions were taken at the start of the exposure
and weekly thereafter. The determination of sorafenibtosylate in the test solutions was carried
out by HPLC/MS.
The endpoint survival was statistically evaluated by the computer program
TOXRat Professional XT, length and weight was evaluated by PROVANTIS (NOEC, LOEC)
and TOXRat Professional XT (ECx).
Then analytical results gave mean measured concentrations of 1, 2.4 and 14.8 µg/L.
Hatching and short-term survival (day 5 ph) was not affected by the test substance while
mortality of the larvae (day 28 ph) increased clearly with increasing concentration of
sorafenibtosylate. Additionally, the growth of the treated fish decreased with increasing
concentration of the test substance.
A significant substance-related effect on growth and survival of the treated fish compared to the
control could be observed. The NOEC (survival) was <1.0 ¿g/L and the LOEC (survival) was
¿1.0 ¿g/L, the EC10 for survival was 0.17 ¿g/L. The NOEC for length and weight was 2.4 ¿g/L,
the LOEC was 14.8 ¿g/L. The EC10 was 0.47 ¿g/L for weight and 0.32 ¿g/L for length.
Accordingly, 0.17 ¿g/L was the toxicity threshold for sorafenib tosylate.
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