Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 607-088-5 | CAS number: 224785-90-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- sampling schedule:
control: 0 and 48 h
test concentration: 0 and 48 h - Vehicle:
- no
- Details on test solutions:
- Reconstituted water (so-called 'M4 medium', originally described in Water Research 24 (9): 1157-1167)
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna STRAUS, parthenogenetic females; Strain of Bundesgesundheitsamt Berlin
A population of parthenogenetic females of synchronised age structure was maintained since more than 15 years in the test facility under constant temperature conditions (20 +/- 1°C) at a 16:8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') was partly renewed once a week. The daphnia are exclusively fed with unicellular green algae (Scenedesmus subspicatus CHODAT) 'ad libitum'. Mortalities of parent daphnia during the culture period were recorded daily in a semi-quantitative way. The neonates were separated from their parent daphnia by filtration prior to the acute test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 13.8°dH (=246 mg/L CaCO3)
- Test temperature:
- 20.1°C
- pH:
- 8.0
- Dissolved oxygen:
- 8.5 mg/L (96.3 % saturation)
- Nominal and measured concentrations:
- Nominal: 2 mg/L
Measured concentration at 48 h: 2.8 mg/L - Details on test conditions:
- PRETREATMENT OF THE TEST ITEM:
A stock solution was prepared to give the desired test concentration. To accelarate the solution procedure, a five fold amount of the water solubility (2mg/L) was taken to produce the stock solution (10 mg/L). The substance was weighed into 1 litre of dilution water and treated for one hour with an unltrasonic bath and afterwards stirred for 24 hours on a magnetic stirrer. Finally undissolved particles of the substance were removed by filtration.
TEST SYSTEM:
Test vessels: 50 mL glass beakers holding 10 neonates in 20 mL of test medium
Experimental design: 1 test concentration plus 1 control; 10 neonates per vessel, 2 replicates per concentration/control; no feeding during the exposure period
Photoperiod: 16 h light : 8 h dark
Temperature: mean +/- 1°C in the temperature range 18 - 22°C
Aeration: none
Test concentration: 2 mg/L nominal
Medium renewal: none - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 2.8 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The results are expressed in terms of nominal concentrations (24h) and in terms of mean measured concentrations (48h). Measured concentrations at 0 and at 48h of exposure correspond to 140% of the nominal value.
- Validity criteria fulfilled:
- yes
- Remarks:
- (- The immobilisation and other abnormalities in the controls < 10% by the end of the test. - The dissolved oxygen concentration remained above 3 mg/L throughout the exposure period.- concentration of test substance shall maintain >= 80% during test.)
- Conclusions:
- For the substance a 48h-EC0 of 2.8 mg/L (measured) was obtained.
- Executive summary:
In order to test acute toxicity to invertebrates of the substance, Daphnia magna was exposed to the test solution of 1 nominal concentration of the test substance (2 mg/L) and blank control solution for a period of 48 h under static conditions. The method used was the EU Method C.2 (Acute Toxicity for Daphnia). Mobility and visible abnormalities were recorded at 24 and 48 h. The measured concentrations at 48 hours was 2.8 mg/L. No toxic effect and no abnormal behaviour was observed (up to the water solubility under exposure conditions). This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute Daphnia study.
Reference
Description of key information
Using the EU Method C.2 (Acute Toxicity for Daphnia) a 72h-EC0 of 2.8 mg/L (measured) was determined. No toxic effect and no abnormal behaviour to Daphnia magna was observed (up to the water solubility under exposure conditions).
Key value for chemical safety assessment
Additional information
In order to test acute toxicity to invertebrates of the substance, Daphnia magna was exposed to the test solution of 1 nominal concentration of the test substance (2 mg/L) and blank control solution for a period of 48 h under static conditions. The method used was the EU Method C.2 (Acute Toxicity for Daphnia). Mobility and visible abnormalities were recorded at 24 and 48 h. The measured concentrations at 48 hours was 2.8 mg/L. No toxic effect and no abnormal behaviour was observed (up to the water solubility under exposure conditions). This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute Daphnia study.
The substance has been tested up to the water solubility under exposure conditions. In rage finding tests the maximum of water solubility of the substance under exposure conditions was determined to be about 2 mg/L. To achieve this concentration, the five fold amount of the substance (10mg/L) was weighed in. In the report of this study a water solubility of the substance of 7 mg/L was presented. This value has been determined within a GLP study. The medium in the respective study differs from the test medium used in the presented study concerning the daphnia toxicity.
The results of the main test as well as those of the range finding test clearly demonstrated that under exposure conditions the maximum water solubility is lower. If a substance is tested at the maximum water solubility, under exposure conditions slight fluctuations of the respective concentrations are to be expected. The observed difference between the nominal concentration of 2 mg/L and the mean measured concentration of 2.8 mg/L can be explained by this and is seen as negligible.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
