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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
Deviations:
no
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Deviations:
no
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
other: mixed population of aquatic microorganisms
Details on inoculum:
Source: secondary effluent of a domestic sewage treatment plant (STP Odenthal, Germany)
Date of collection: 2000-01-21
Preconditioning: separation of coarse particles by filtration; aeration of mineral medium plus inoculum (filtrate) for 6 days
Effluent concentration in reaction mixture: 5 mL/L
Duration of test (contact time):
28 d
Initial conc.:
4.1 mg/L
Based on:
test mat.
Details on study design:
Pretreatment of test substance:
The test substance was weighed out on aluminium foil. This substance inclusive the aluminium foil was added to the test vessel.

Exposure conditions:
Incubation time: 28 d
Incubation temperature: 20 - 21°C
Reference substance:
benzoic acid, sodium salt
Remarks:
Purity: 99%; initial concentration: 2.9 mg/l
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d
Value:
1 703 mg O2/g test mat.
Results with reference substance:
77 % degradation of benzoic acid, sodium salt within 14 days, and 81 % degradation within 28 days.

The test substance is not toxic to bacteria.

Validity criteria fulfilled:
yes
Remarks:
77 % degradation of the reference compound benzoic acid, sodium salt within 14 days
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
With 0 % degradation within 28 days the substance is considered as not readily biodegradable.
Executive summary:

Using the EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test) 0 % degradation within 28 days was observed for the substance. Therefore, the substance is considered as not readily biodegradable.

Description of key information

With 0 % degradation within 28 days the substance is considered as not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

Using the EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test) 0 % degradation within 28 days was observed for the substance. Therefore, the substance is considered as not readily biodegradable.