Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 941-492-5
CAS number: -
A Respiration Inhibition Test with activated sludge according to
OECD Guideline No. 209 was carried out with the test item Sepisolfast
yellow MG-F (batch number:407940) from 2017-04-18 to 2017-04-19, with
the definitive exposure phase on 2017-04-19 at the test facility.
The test system was activated sludge of the municipal treatment
plant of 31137 Hildesheim, Germany. The test was carried out under
static conditions with the test item concentrations 4 - 16 - 64 - 256 -
The respiration rates of the control, reference and test item
replicates were measured after a contact time of three hours, and the
inhibitory effects of the test and reference item were determined in
comparison to the control respiration rates. The mean inhibition of
respiration for the test item replicates ranged from -2 % to 57 %.
In order to check the activity of the test system and the test
conditions a reference test was carried out with copper (II) sulphate
pentahydrate as reference item and the reference toxicity was
determined. The EC50-value for the reference item was 109
Table 1 : NOEC and EC-Values with Confidence Interval of
Sepisol fast yellow MG-F after 3 h exposure time, Inhibition of Total
Confidence interval P = 95 [%][mg/L]
46.0 – 53.8
60.0 – 65.4
103 – 127
*) No statistically significant
inhibition (P < 0.001), ANOVA, Dunnett`s Method
Conclusion: the NOEC of Sepisol fast yellow MG-F is 16 mg/L and
the EC50 is 115 mg/L.
Activated sludge, Respiration Inhibition test: EC50 (3h): 115
mg/L corrected to 75 mg/L (water solubility) (OECD TG 209; static).
The EC50 (3 hours) was 3 mg/L with 95% confidence limits of 103 -127
mg/L. The inhibition range from 2% to 57%. However, as the substance has
a water solubility 75 mg/L, the EC50 for the test substance has been
considered to be 75 mg/L.
Other data (non corrected):
The EC10 (3 hours) was 50.2 mg/L (measured concentrationx) with 95%
confidence limites of 46 -53.8 mg/L.
The NOEC (3 hours) was 16 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again