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EC number: 700-579-6 | CAS number: -
T = 24 hours
Immobilization rate %
T = 48 hours
The short-term toxicity (48 hours) on invertebrate (Daphnia magna) of the test item Sepisol Fast Blue 85219 was assessed by determining the nominal effective concentration (EC 50%) at which 50 % of the test animals are immobilized. The method followed was the standard NF EN ISO 6341 under static condition.
EC50 (48h) values and associated confidence limits were calculated using a Log-Probit statistical model (software Toxcalc) and NOEC was worked out by using the Bonferroni t-test (software Toxcalc).
Test System: A stock solution (14 mg/mL) of the test substance was prepared in ultra-pur water.
Immobilisations and viable abnormalities were recorded at 24 and 48 hours as well as pH and dissolved oxygen.
Validity criteria were fulfilled and study was considered as valid..
A preliminary assay was performed at the following concentration: 12.6 - 4.9 - 1.4 - 0.5 - 0.014 - 0.0049 mg/L
Test Levels: Test concentrations included control, 0.18 - 0.11 - 0.063 - 0.036 - 0.021 - 0.011 - 0.007 - 0.0042 - 0.0025 - 0.0014 mg/L. Twenty daphnia were used per test group. 3 replicates were performed.
24-h EC50=0.012 mg/L (IC 95%: 0.0095-0.015)
48-h EC50=0.0057 mg/L (IC 95%: 0.0047-0.0069)
48-h NOEC= 0.0025 mg/mL
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