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EC number: 203-402-6 | CAS number: 106-48-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions (no analytical purity reported; no E.coli strain tested)
Data source
Reference
- Reference Type:
- publication
- Title:
- Salmonella Mutagenicity Test Result for 250 Chemicals.
- Author:
- Haworth S, Lawlor T, Mortelmans K, et al.
- Year:
- 1 983
- Bibliographic source:
- Environmental Mutagenesis Supplement 1:3 - 142
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- no analytical purity reported; no E.coli strain tested
- GLP compliance:
- no
- Remarks:
- prior to GLP
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4-chlorophenol
- EC Number:
- 203-402-6
- EC Name:
- 4-chlorophenol
- Cas Number:
- 106-48-9
- Molecular formula:
- C6H5ClO
- IUPAC Name:
- 4-chlorophenol
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Cofactor supplemented post-mitochondrial fraction (S9 mix), prepared from livers of rats and hamsters treated with Aroclor 1254.
- Test concentrations with justification for top dose:
- 10, 33.3, 100, 333.3, 1000 µg/plate without and with metabolic activation
- Vehicle / solvent:
- - Vehicle/solvent used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- +S9: 1.0 µg/plate 2-aminoanthracene (2-AA) for TA 98, TA 100, 2.5 µg/plate for TA 1535 and TA 1537
- Positive control substance:
- other: -S9: 5 µg/plate 4-Nitro-ophenylenediamine (NOPD) for TA 98, 1 µg/plate Sodium azide (SA) for TA100 and TA 1535 and 50 µg/plate 9-Aminoacridine (9-AA) for TA 1537
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
- Salmonella strains TA98, TA100, TA 1535 and TA1537 were used in a modification of the preincubation test of Yahagi et al., 1975 (Mutagenicity of carcinogenic azo dyes and their derivatives. Cancer Lett 1:91-96).
- The preincubation procedure was selected because of reports that it was no less sensitive than the plate test, and was more effective than the plate test for various chemicals such as aliphatic nitrosamines, pyrrolizidine alkaloids, and volatile chemicals.
DURATION
- Preincubation period: 20 min
- Exposure duration: 48 h at 37 °C
NUMBER OF REPLICATIONS: three replicates each in two independent experiments
DETERMINATION OF CYTOTOXICITY
- Method: thinning or absence of the bacterial lawn. - Evaluation criteria:
- The test substance may be considered positive in this test system if the following criteria are met: For the test substance to be considered mutagenic, a reproducible dose related increase, whether it is twofold over background or not.
- Statistics:
- Mean values and standard deviation were calculated.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- yes 1000 µg/plate TA 100, +S9
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES: The highest concentration analysed was selected on the solubility of the test substance in cell culture medium.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1. Test results of the experiment (preincubation)
With or without S9-Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 3 plates ± Standard deviation) |
|||
Base-pair substitution type |
Frameshift type |
||||
TA 100 |
TA1535 |
TA1537 |
TA98 |
||
– |
0.0 |
89 ± 5.5 |
19 ± 3.2 |
13 ± 2.1 |
23 ± 3.4 |
– |
10.0 |
62 ± 9.8 |
10 ± 2.3 |
5 ± 0.6 |
18 ± 3.3 |
– |
33.3 |
65 ± 1.0 |
11 ± 3.3 |
8 ± 2.0 |
20 ± 4.1 |
– |
100.0 |
55 ± 4.3 |
14 ± 2.0 |
7 ± 3.5 |
18 ± 3.4 |
– |
333.3 |
62 ± 3.0 |
8 ± 2.6 |
6 ± 0.6 |
17 ± 1.5 |
– |
1000.0 |
47 ± 10.0 |
9 ± 1.8 |
8 ± 0.6 |
3 ± 2.0 |
Positive controls, –S9 |
Name |
SA |
SA |
4NQO |
9AA |
Mean No. of colonies/plate (average of 3 ± SD) |
494 ± 8.4 |
484 ± 9.2 |
697 ± 292.4 |
615 ± 9.0 |
|
+ (rat) |
0.0 |
73 ± 5.2 |
4 ± 1.0 |
5 ± 0.3 |
24 ± 4.4 |
+ (rat) |
10.0 |
78 ± 2.8 |
6 ± 0.7 |
5 ± 0.6 |
28 ± 1.8 |
+ (rat) |
33.3 |
74 ± 2.8 |
5 ± 1.3 |
5 ± 0.6 |
30 ± 1.7 |
+ (rat) |
100.0 |
94 ± 9.0 |
6 ± 1.5 |
5 ± 1.7 |
30 ± 2.8 |
+ (rat) |
333.3 |
73 ± 3.8 |
10 ± 1.2 |
6 ± 1.9 |
29 ± 5.6 |
+ (rat) |
1000.0 |
T |
9 ± 0.7 |
1 ± 7 |
14 ± 1.5 |
Positive controls, +S9 (rat) |
Name |
2AA |
2AA |
2AA |
2AA |
Mean No. of colonies/plate (average of 3 ± SD) |
877 ± 45.1 |
191 ± 28.3 |
244 ± 10.5 |
956 ± 33.1 |
|
+ (hamster) |
0.0 |
97 ± 10.5 |
15 ±1.8 |
33 ± 1.8 |
41 ± 4.5 |
+ (hamster) |
10.0 |
77 ± 1.5 |
8 ± 1.9 |
8 ± 2.8 |
28 ± 0.7 |
+ (hamster) |
33.3 |
70 ± 8.1 |
11 ± 3.5 |
9 ± 3.0 |
27 ± 4.5 |
+ (hamster) |
100.0 |
75 ± 7.7 |
11 ± 1.7 |
10 ± 1.3 |
28 ± 0.9 |
+ (hamster) |
333.3 |
75 ± 6.7 |
14 ± 3.5 |
8 ± 1.5 |
26 ± 0.3 |
+ (hamster) |
1000.0 |
42 ± 12.0 |
8 ± 0.6 |
7 ± 1.3 |
28 ± 0.6 |
Positive controls, +S9 (ha mster) |
Name |
2AA |
2AA |
2AA |
2AA |
Mean No. of colonies/plate (average of 3 ± SD) |
1215 ± 16.4 |
458 ± 3.3 |
245 ± 7.5 |
1397 ± 41.3 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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