Triethyl phosphite

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Adopted according to OECD SIDS (publicly available peer reviewed source). Original document not available.

Data source

Reference

Reference 1

Reference Type:

secondary source

Title:

SIDS Initial Assessment Report For SIAM 16 (Triethyl Phosphite), 2003 Paris.

Author:

OECD

Year:

2003

Bibliographic source:

UNEP Publications

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Version / remarks:

(adopted 1983)

Deviations:

no

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Version / remarks:

(adopted 1997)

Deviations:

yes

Remarks:

Only 4 tester strains used. 2-Aminoanthracene was used as the sole positive control in the presence of S9-Mix.

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Triethyl phosphite

EC Number:

204-552-5

EC Name:

Triethyl phosphite

Cas Number:

122-52-1

Molecular formula:

C6H15O3P

IUPAC Name:

triethyl phosphite

Details on test material:

Purity: 99.45%

Method

Target gene:

HIS operon

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

Rat liver S9-mix from Aroclor 1254 induced rats.

Test concentrations with justification for top dose:

First test: 20, 100, 500, 2500, 12500 µg/plate.
Repeated test: 775, 1550, 3100, 6200, 12400 µg/plate.

Vehicle / solvent:

Dimethylformamide

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: without S-9: sodium azide (10 µg/plate), nitrofurantoin (0.2 µg/plate), 4-nitro-1,2-phenylene-diamine (10 µg/plate), with S-9: 2-aminoanthracene (3 µg/plate)

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation)

NUMBER OF REPLICATIONS: no data

Evaluation criteria:

A reproducible and dose-related increase (i.e. greater than twice the negative control count) in mutant counts for at least one strain was considered a positive result.

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

>775 µg/plate

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

valid

Additional information on results:

None of the tested strains showed a dose-related and biologically relevant increase in mutant counts over those of the negative controls, both with and without the metabolic activation.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative