o-fluoro-α,α,α-trichlorotoluene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.3 (Acute Toxicity (Dermal))

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

o-fluoro-α,α,α-trichlorotoluene

EC Number:

207-692-5

EC Name:

o-fluoro-α,α,α-trichlorotoluene

Cas Number:

488-98-2

Molecular formula:

C7H4Cl3F

IUPAC Name:

1-fluoro-2-(trichloromethyl)benzene

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

unchanged (no vehicle)

Duration of exposure:

14 days

Doses:

The test article was applied at a dose of 2000 mg/kg b.w. evenly on the intact skin with a syringe and covered wita asemi-occlusive dressing.
Application volume/kg b.w.: 2.0 mL

No. of animals per sex per dose:

5 males
5 females

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

No deaths occurred during the study.

Clinical signs:

The following local alteration of the skin at the application site was noted:
2000 mg/kg
Scales on the back (5/5)*
General erythema on the back (4/5)
Crusts on the back (1/0)

In addition, emaciation was observed in 2 male and 3 female rats.
* (/) = number of male/female animals with local skin alterations.

Body weight:

Four male and five female animals lost weight (between 0.9% and 8.6%) during the first week of observation. They recovered during the second observation week.

Other findings:

Macroscopic findings:
No macroscopic organ findings were noted at necropsy.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The LD50 of test substance after single dermal administration to rats of both sexes, observed over a period of 14 days, could not be estimated as no death occurred.
LD50 is > 2000 mg/kg b.w.