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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to a guideline study and current standards but with limited documentation. Restricted to only one sampling time. Information taken from a secondary source (OECD, 2006).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987

Materials and methods

Principles of method if other than guideline:
Comparable to a guideline study but with only one sampling time.
GLP compliance:
not specified
Type of assay:
micronucleus assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Methanol, reagent special grade from Junsei Chemicals Co., no further data.

Test animals

Species:
mouse
Strain:
ICR
Sex:
male

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
No information
Duration of treatment / exposure:
single, 24 hours
Frequency of treatment:
once
Doses / concentrations
Remarks:
Doses / Concentrations:
1050, 2110, 4210 or 8410 mg/kg bw
Basis:
nominal conc.
No. of animals per sex per dose:
6 per dose level and positive control (TEM)
Control animals:
yes, concurrent no treatment
Positive control(s):
Yes, TEM

Examinations

Tissues and cell types examined:
Bone marrow

Results and discussion

Test results
Sex:
male
Genotoxicity:
negative
Toxicity:
not specified
Vehicle controls validity:
not specified
Negative controls validity:
not examined
Positive controls validity:
valid

Any other information on results incl. tables

There was no methanol-related increase in the induction of micronuclei: In treated groups, the mean MN rate varied from 0.5 to 2.2/1000 vs. 1.5/1000 in the neg. control and 45/1000 in the pos. control group.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative
There were no methanol-related increases in the induction of micronuclei in treated groups.