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Short-term toxicity to aquatic invertebrates

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no observable effect at highest concentration achievable

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The acute toxicity of (2-Ethoxy-1-methyl-2-oxoethyl)-5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-nitrobenzoate to Daphnia magna STRAUS was studied under semi-static conditions in accordance with the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test' (adopted April 13, 2004) which is in most parts equivalent to the EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 2 'Acute toxicity for Daphnia'. Under semi-static test conditions, the invertebrates were exposed to a range of concentrations, nominally 0.25, 0.5 and 1 mg/L of (2-Ethoxy-1-methyl-2-oxoethyl)-5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-nitrobenzoate in water. The swimming ability and the immobilization after 24 and 48 hours of exposure were determined. The EC50was > 0.007 mg/L for 24 h and > 0.003 mg/L for 48 h incubations. No effect on Daphnids was observed at any concentration including the highest concentrations achievable.