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EC number: 700-674-2 | CAS number: 147993-65-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian cell study: DNA damage and/or repair
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 Mar 2017 to 10 Aug 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 489 (In vivo Mammalian Alkaline Comet Assay)
- Version / remarks:
- July 29, 2016
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- mammalian comet assay
Test material
- Reference substance name:
- 4,4'-methylenediphenyl diisocyanate
- EC Number:
- 202-966-0
- EC Name:
- 4,4'-methylenediphenyl diisocyanate
- Cas Number:
- 101-68-8
- Molecular formula:
- C15H10N2O2
- IUPAC Name:
- 1,1'-methylenebis(4-isocyanatobenzene)
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- - Name of the test item (as cited by study report): 4,4’-Diphenylmethane Diisocyanate (MDI)
- Batch No.: P4DB005186
- Purity: 98.89%.
- Retest date: 30 Jun 2017
- Appearance: White solid
Test animals
- Species:
- rat
- Strain:
- Wistar
- Details on species / strain selection:
- Crl:WI (Han)
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories
- Age at study initiation: Approximately 7 weeks
- Weight at study initiation: 168g - 216 g
- Assigned to test groups randomly: yes, the animals judged suitable for assignment to the study were selected for use in a computerized randomization procedure based on body weight stratification in a block design.
- Housing: Upon arrival, all animals were housed 2 to 3 per cage in clean, solid bottom cages containing ground corncob bedding material (Bed O’Cobs®; The Andersons, Cob Products Division, Maumee, OH). Enrichment devices were provided to all animals as appropriate throughout the study for environmental enrichment and to aid in maintaining the animals’ oral health, and were sanitized weekly.
- Diet: The basal diet used in this study, PMI Nutrition International, LLC, Certified Rodent LabDiet® 5002 (meal), is a certified feed with appropriate analyses performed by the manufacturer and provided to Charles River. The basal diet was provided ad libitum throughout the study, except during exposure periods.
- Water: Reverse osmosis-treated (on-site) drinking water, delivered by an automatic watering system, was provided ad libitum throughout the study, except during exposure periods. Municipal water supplying the facility was analysed for contaminants according to SOPs.
- Acclimation period: All animals were housed for an 14-day acclimation. During acclimation, each animal was observed twice daily for mortality and changes in general appearance or behaviour.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21.3 – 22.6
- Humidity (%): 37.9 – 46.5
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Vehicle:
- Filtered air
- Details on exposure:
- TYPE OF INHALATION EXPOSURE: nose only
EXPOSURE SYSTEMS
Exposures were conducted using a 2 tier (7.9-L) stainless steel, conventional nose-only exposure systems (CNOS), with grommets in the exposure ports to engage animal holding tubes. Four dedicated exposure systems were used: 1 for the filtered-air control group and 1 for each of the test substance groups. Air supplied to the nose-only systems was provided from the Charles River Inhalation Department breathing quality, in-house compressed air source. All nose-only system exhaust passed through a Solberg canister filter prior to entering the facility exhaust system, which consists of redundant exhaust blowers preceded by activated-charcoal and HEPA-filtration units.
CHAMBER DESCRIPTION
All animals were housed in a normal animal colony room during non-exposure hours. Prior to the exposure, the animals selected for exposure were placed into nose-only restraint tubes in the colony room and transported to the exposure room. Animals were then placed on the nose-only systems and exposed for the requisite duration. After being transported to the exposure room, the animals assigned for exposure were held in restraint tubes for approximately 23 to 26 minutes before the initiation of exposure to allow the animals breathing rates to return to normal baseline values. Food and water were withheld during nose-only restraint tube acclimation and during the exposure period. Oxygen content of the exposure atmospheres was measured during the method development phase of the study using a Dräger PAC III equipped with a calibrated oxygen sensor (Serial No.ERRH-0148, Draeger Safety Inc.; Pittsburgh, PA) and was 20.9% for all groups. Due to the local weather conditions near/at the start of animal exposures, the compressed air used was not as dry as expected. This caused the average humidity for Group 4 to be higher than the protocol-specified target range. The humidity was kept as low as practical to minimize test substance dimer formation.
CONTROL EXPOSURE SYSTEM (EXPOSURE SYSTEM 1)
The control exposure system (Group 1) was operated as follows: dry air was added to the CNOS inlet using a regulator and controlled using a rotameter-type flowmeter.
TEST SUBSTANCE EXPOSURE SYSTEMS (EXPOSURE SYSTEMS 2 TO 4)
A dedicated generation system was used for each test substance exposure system and was operated as follows: A liquid droplet aerosol atmosphere of the test substance was generated using a single-jet Collison nebulizer filled with an appropriate amount of test substance, and heated to approximately 80°C to melt and maintain the test substance in liquid form. Using a regulator, dry, compressed air at a controlled pressure was supplied to the generation port of each nebulizer to affect aerosolization of the test substance. The air entering each nebulizer was also heated. The resulting aerosol from each nebulizer was delivered to a mixing plenum, where it mixed with additional dry, dilutionair. In order to permit reduction of the exposure concentration, a portion of the aerosol output from each nebulizer and/or mixing plenum was directed toward facility exhaust. The remaining aerosol within each mixing plenum was delivered to the “T”-fitting located prior to the inlet of the respective nose-only exposure system, where it was mixed with additional dry air prior to entering each CNOS to permit further reduction of the aerosol concentration.
NOMINAL EXPOSURE CONCENTRATIONS
Nominal exposure concentrations were not calculated for this study due to the nature of each aerosol generation system, where a large portion of the test substance aerosol was removed prior to the final dilution to the target concentration. However, the amount of test substance used during the exposure was calculated by weighing each test substance nebulizer prior to and postexposure.
ACTUAL EXPOSURE CONCENTRATIONS
Aerosol exposure concentrations were measured using standard gravimetric methods. Sample flow was measured using a mini-Buck calibrator. The mass concentration in (mg/m3) was calculated from the filter weight difference divided by the sample volume. Samples were collected at least 4-6 times during each exposure for Exposure Systems 2 to 4 and once for Exposure System 1. Each test substance exposure atmosphere was continuously monitored for aerosol concentration using a light scattering type real time aerosol monitor. These instruments were not intended to define exposure concentration, but were to provide exposure personnel with
an indication of approximate aerosol concentration for guidance in making appropriate system adjustments and achieving the most stable exposure concentration possible.
AEROSOL PARTICLE SIZE MEASUREMENT
Aerosol particle size measurements were conducted using a 7-stage brass cascade impactor. Aerosol particle size measurements were conducted once during the exposure period for each test substance group (Groups 2 to 4). Samples were collected at approximately 1.8 to 1.9 LPM for 360, 150, and 60 minutes for Groups 2,3, and 4, respectively. Following sample collection, substrates were re-weighed and the particle size was calculated based on the impactor stage cut-offs. The particle-size was expressed as the mean aerodynamic diameter (MMAD) in microns and the geometric standard deviation (GSD). See 'Any other information on materials and methods incl. tables' for a summary of the aerosol particle size for each test substance-treated group.
POSITIVE CONTROL SUBSTANCE PREPARATION
The positive control substance formulation was prepared on each day of dose administration (Study Days 0 and 1) as a weight/volume (EMS/0.9% saline) mixture.
JUSTIFICATION OF DOSE LEVELS
Doses were selected based on the outcome of the dose-range finding study (see cross-referenced acute inhalation toxicity record, see section 7.2.2) and other supporting studies (See Acute Toxicity: Inhalation; Pauluhn, 2000; Hotchkiss, 2017) while taking the recommendations in OECD TG 489 to consider cytotoxicity in setting maximum tolerated concentration (MTC). In the range-finding study, Wistar rats were exposed to 3.2, 7.7, and 11.9 mg MDI/m3 for 6 h and biomarkers for local cytotoxicity were analysed 1 h after the exposure and 18 h following completion of exposure. Results demonstrate both concentration- and time-dependent effects of inhaled 4,4’-MDI aerosol on biomarkers of exposure. Compared to control rats, dose dependent increases associated with macrophage activation (β-glucuronidase activity), inflammation (neutrophil infiltration and total protein)) at ≥3.2 mg MDI/m3, apoptosis (Annexin V activity; ≥ 7.7 mg MDI/m3), and necrosis (LDH; ≥ 11.9 mg MDI/ m3) were noted. This is supported by other supporting studies that demonstrate that acute exposure during 6 h to a concentration of 10-12 mg/m3 resulted in significant increases in biomarkers for inflammation, cytotoxicity/apoptosis, and macrophage activation (See Acute Toxicity: Inhalation; Pauluhn, 2000; Hotchkiss, 2017). Moreover, concentrations > 20 mg/m3 (for 3 hr) were sufficient to induce cytotoxicity induced DNA damage, but 10 mg/m3 (for 6h) were not (Sutter, 2016). Taken together, the MTC was considered 11.9 mg/m3 on the basis of marked increases in inflammation, apoptosis/necrosis, and cytotoxicity. The remaining 2 concentrations be appropriately spaced as to demonstrate a dose response with the lowest producing little to no toxicity. In the range-finding study, concentrations of 3.2 and 7.7 mg/m3 resulted in mild to moderate concentration-dependent changes as measured by inflammation and macrophage activity (β-glucuronidase). As the midconcentration, 5 mg/m3 is selected as it is anticipated to induce mild to moderate cytotoxic effects. As the low-concentration, 2 mg/m3 is selected as it is expected to result in little to no cellular toxicity. The selection of concentrations is in agreement with the recommendations described in OECD TG 489. - Duration of treatment / exposure:
- 6 hours
- Frequency of treatment:
- One exposure period
- Post exposure period:
- Six rats/group terminated on Study Day 0 within approximately 1 hour postexposure (Groups 1–4), on Study Day 1 at approximately 18 hours postexposure (Groups 1–4), or on Study Day 1 between 2 and 4 hours after the second dose of EMS (Group 5); bronchoalveolar lavage fluid (BALF), liver, and glandular stomach collected and processed for in vivo comet assay evaluation from 6 animals/sex/group at each time point; bronchoalveolar lavage and fluid analysis performed on all animals; carcasses and remaining tissues discarded.
Doses / concentrationsopen allclose all
- Dose / conc.:
- 2 mg/m³ air (nominal)
- Remarks:
- Analytical concentration: 2.5 mg/m³
- Dose / conc.:
- 5 mg/m³ air (nominal)
- Remarks:
- Analytical concentration: 4.9 mg/m³
- Dose / conc.:
- 11 mg/m³ air (nominal)
- Remarks:
- Analytical concentration: 12 mg/m³
- No. of animals per sex per dose:
- -Test groups (Group 2-4): 12 males
- Vehicle control (Group 1): 12 males
- Positive control (Group 5): 6 males - Control animals:
- yes, concurrent vehicle
- Positive control(s):
- The positive control substance, ethyl methanesulfonate (EMS; CAS 62-50-0), was administered via oral gavage to rats in Group 5 at a dose of 200 mg/kg/day on Study Days 0 and 1. The vehicle used in preparation of the positive control substance formulation was 0.9% sodium chloride for injection. The positive control substance was stored refrigerated (2°C to 8°C), purged with nitrogen, and was considered stable under these conditions. The route of administration of the positive control substance (oral gavage) was chosen based on previous data in which BioReliance demonstrated that orally administered EMS produced a significant increase in DNA damage (comet response) in the BAL cells, liver, and glandular stomach.
Examinations
- Tissues and cell types examined:
- Samples of the BALF, liver and stomach were collected from all animals and processed for the comet assay evaluation.
BAL cells have been used have been regularly used in the assessment of pulmonary genotoxicity after inhalation or instillation of various substances. Pulmonary macrophages are predominant in the BAL fluid, usually one of the first cell types to come into contact with aerosols and thus represent the site of contact in the lung; this is especially relevant for very reactive substances such as 4,4’-MDI. The use of BAL cells, versus using minced lung tissue, reduces the dilution of effects from unexposed cells that occurs when for instance alveolar epithelial cells are isolated. It is technically difficult to harvest and sort different cell types from minced lung tissue. Harsh treatment often results in high background damage and a major share of cells in minced lung tissue consist of haematopoetic cells. This means that only a relatively large induction of DNA damage would be identified by the sensitive Comet Assay. Importantly, during development of the historical control database in the performing laboratory in a preliminary study, it was observed that the positive control substance EMS rapidly distributes from the lymph system to the BAL to induce DNA damage. This was confirmed during the main study and further demonstrates the value of BAL cells as an appropriate tissue to be examined. Liver was included since it is the site of primary metabolism; and it was included to investigate systemic (as opposed to local) genotoxicity. Glandular stomach was included due to possible exposure after clearance of 4,4’-MDI via the mucociliary escalator. - Details of tissue and slide preparation:
- MACROSCOPIC EXAMINATION
All animals were anesthetized by isoflurane inhalation and euthanized by exsanguination. The animals were not fasted overnight prior to the scheduled euthanasia, and a gross necropsy was not performed. Immediately following euthanasia, BAL was performed on all animals and processed. At the time of euthanasia, samples of the following tissues were collected and placed in 10% neutral-buffered formalin. The carcasses and remaining tissues were discarded.
HISTOLOGY AND MICROSCOPIC EXAMINATION
After fixation, protocol-specified tissues were trimmed according to the test laboratory SOPs and the protocol. Trimmed tissues were processed into paraffin blocks, sectioned according to the test laboratory SOPs, mounted on glass microscope slides, and stained with hematoxylin and eosin from all animals at the scheduled necropsies.
BRONCHOALVEOLAR LAVAGE
At the scheduled euthanasia, BAL was performed on the lungs of all animals. The trachea was exposed and a dosing cannula was tied in place in the trachea. The lungs were lavaged 6 times with a lavage solution volume of 25 µL/gram body weight (based on the most recent body weights) of room temperature Hank’s Balanced Salt Solution (sterile) without calcium, magnesium, or phenol red, up to a maximum volume of 4 mL (per lavage). The BALF from the first and second lavages was recovered after remaining in the lung for approximately 1 minute and placed into a 15-mL polypropylene centrifuge tube after the volume was recorded. For the third through sixth lavages, the recovered volumes were recorded and the BALF was collected into a single polypropylene centrifuge tube. Recovered BALF was stored on ice until processed. The following parameters were evaluated from the BAL fluid or cell pellet: Alkaline phosphatease (ALP), Annexin V, ß-glucuronidase, cell differential (cytology), lactate dehydrogrenase (LDH) and total protein (see cross-referenced acute inhalation study, section 7.2.2).
BRONCHOALVEOLAR LAVAGE FLUID PROCESSING
The BALF was isolated in a refrigerated centrifuge and the supernatant fluid from the first and second lavages was transferred to a sealable vial and stored on an ice bath until used for analysis of BAL clinical chemistry (alkaline phosphatase, lactate dehydrogenase, and total protein) and/or transferred to the internal immunotoxicology department for analysis of beta. The supernatant fluid from the third through sixth lavages was decanted and discarded. The cell pellets obtained from the first and second lavages or third through sixth lavages were pooled separately and retained. The cell pellet from the first and second lavages was resuspended in cold Roswell Park Memorial Institute (RPMI) media with 10% fetal calf serum, and this cell suspension was also used to resuspend the cell pellet from the third through sixth lavages. Total cell counts were obtained using a hemocytometer with cell viability assessed by trypan blue exclusion. A portion of the cell suspensions were transferred into Dulbecco’s Phosphate-Buffered Saline (DPBS; with calcium and magnesium) for future processing and analysis of Annexin V (see cross-referenced acute inhalation study, section 7.2.2). The remainder of the cell suspension was centrifuged again and a portion of the cell pellet was used for the comet assay.
TISSUE SELECTION FOR COMET ASSAY
A section of the liver was placed in 3 mL of chilled mincing solution (Hanks’ balanced salt solution with EDTA and DMSO), then minced with fine scissors to release the cells. A section of the glandular stomach was placed in 1 mL of chilled mincing solution then scraped using a plastic spatula to release the cells. The cell suspensions were strained into a pre-labeled conical polypropylene tube through a Cell Strainer and were kept on wet ice during preparation of the slides. The remaining cell pellet from the bronchoalveolar lavage was mixed with chilled mincing solution. The mixture was kept on wet ice during the preparation of the slides.
PREPARATION OF COMET SLIDES
- Preparation of slides: From each liver, stomach and BAL cell suspension, an aliquot of 2.5 µL, 7.5 µL and 7.5 µL, respectively, were mixed with 75 µL (0.5%) of low melting agarose. The cell/agarose suspension was applied to microscope slides commercially available pre-treated multi-well slides. Commercially purchased multi-well slides were used and these slides have 20 individual circular areas, referred to as wells in the text below. The slides were kept refrigerated for at least 15 minutes to allow the gel to solidify. At least two Trevigen, Inc. 20-well slides were prepared per animal per tissue. Slides were identified with a random code that reflects the study number, group, animal number, and organ/tissue. Three wells were used in scoring and the remaining wells were designated as a backup. The backup slides/wells may be used in additional scoring, if deemed necessary. Following solidification of agarose, the slides were placed in jars containing lysis solution.
- Lysis: Following solidification of agarose, the slides were submerged in a commercially available lysis solution supplemented with 10% DMSO on the day of use. The slides were kept in this solution at least overnight at 2-8 °C.
- Unwinding: After cell lysis, slides/wells were washed with neutralization buffer (0.4 M tris hydroxymethyl aminomethane in purified water, pH ~7.5) and placed in the electrophoresis chamber. The chamber reservoirs were slowly filled with alkaline buffer composed of 300 mM sodium hydroxide and 1 mM EDTA (disodium) in purified water. The pH was > 13. All slides remained in the buffer for 20 minutes at 2-10 °C and protected from light, allowing DNA to unwind.
- Electrophoresis: Using the same buffer, electrophoresis was conducted for 30 minutes at 0.7 V/cm, at 2-10 °C and protected from light. The electrophoresis time was constant for all slides.
- Neutralization: After completion of electrophoresis, the slides were removed from the electrophoresis chamber and washed with neutralization buffer for at least 10 minutes. The slides (gels) were then dehydrated with 200-proof ethanol for at least 5 minutes, then air dried for at least 2 hours and stored at room temperature with desiccant.
- Staining: Slides were stained with a DNA stain (i.e., Sybr-gold™) prior to scoring. The stain solution was prepared by diluting 1 µL of Sybr-gold™ stain in 15 mL of 1xTBE (tris-boric acid EDTA buffer solution).
METHOD OF ANALYSIS
Three wells per organ/animal/treatment were used for the first five animals in each group/time point. The wells from the 6th animal in each group/time point will only be scored due to loss of any animals or rejection of the samples (on quality grounds) after consultation between the sponsor and study director. Fifty randomly selected, non-overlapping cells per slide/well were scored resulting in a total of 150 cells evaluated per animal for DNA damage using the fully validated automated scoring system Comet Assay IV from Perceptive Instruments Ltd. (UK).
The following endpoints of DNA damage were assessed and measured:
- Comet Tail Migration; defined as the distance from the perimeter of the Comet head to the last visible point in the tail.
- % Tail DNA; (also known as % tail intensity or % DNA in tail); defined as the percentage of DNA fragments present in the tail.
- Tail Moment (also known as Olive Tail moment); defined as the product of the amount of DNA in the tail and the tail length [(% Tail DNA x Tail Length)/100.
Each slide/well was also examined for indications of cytotoxicity. The rough estimate of the percentage of “clouds” was determined by scanning 150 cells per animal, when possible (percentage of “clouds” was calculated by adding the total number of clouds for all slides scored, dividing by the total number of cells scored and multiplying by 100). The “clouds”, also known as “hedgehogs”, are a morphological indication of highly damaged cells often associated with severe genotoxicity, necrosis or apoptosis. A “cloud” is produced when almost the entire cell DNA is in the tail of the comet and the head is reduced in size, almost nonexistent. “Clouds” with visible gaps between the nuclei and the comet tail were excluded from comet image analysis. - Evaluation criteria:
- CRITERIA FOR DETERMINATION OF A VALID TEST
- The DNA damage data (% tail DNA) in the filtered air control group is expected to be within the historical vehicle control range.
- The mean number of clouds for a tissue from a filtered air control group animal should not exceed 30%.
- The positive control group must be significantly increased relative to the concurrent filtered air control group (p ≤ 0.05).
EVALUATION OF THE TEST RESULTS
- The test substance will be considered to induce a positive response in a particular tissue if the mean % tail DNA (or other parameters of DNA damage) in one or more test substance groups (doses) is significantly elevated relative to the concurrent filtered air control group.
- The test substance will be judged negative for induction of DNA damage if no statistically significant increase in the mean % DNA damage (or other parameters) in the test substance groups relative to the concurrent filtered air control group is observed.
- The historical vehicle control data; a statistically significant increase in the mean % DNA (or other parameters) may not be considered biologically relevant if the values do not exceed the range of historical vehicle control.
- Any statistically significant increase in DNA damage occurring at a cytotoxic dose may not be considered as a positive finding.
- If a dose-response is evident with no statistically significant increase, additional testing, including histopathology evaluation of the tissue, may be considered.
- If criteria for either a positive or negative response are not met, the results may be judged as equivocal. - Statistics:
- In order to quantify the test substance effects on DNA damage, the following statistical analysis was performed:
- The use of parametric or non-parametric statistical methods in evaluation of data was based on the variation between groups. The group variances for % tail DNA generated for the vehicle and test substance groups were compared using Levene’s test (significant level of p ≤ 0.05). If the differences and variations between groups were found not to be significant, a parametric one-way ANOVA followed by a Dunnett’s post-hoc test was performed (significant level of p < 0.05).
- A linear regression analysis was conducted to assess dose responsiveness in the test substance treated groups (p < 0.01).
- A pair-wise comparison (Student’s T-test, p ≤ 0.05) was used to compare the positive control group against the concurrent vehicle control group.
Results and discussion
Test results
- Key result
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- see "Additional information on results"
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- GENOTOXICITY
- No significant differences in group variance was noted for all tissues at 1 hour and 18 hours after exposure. Therefore, the parametric approach, ANOVA followed by Dunnett’s post-hoc analysis, was used in the statistical analysis of data.
- No statistically significant response in the % Tail DNA (DNA damage) was observed in the test substance groups relative to the concurrent filtered air control groups for all tissues at 1 hour and 18 hours after exposure.
- No dose-dependent increase in the % Tail DNA was observed across three test substance doses for all tissues at 1 hour and 18 hours after exposure.
- In the filtered air control groups, % Tail DNA was within the historical vehicle control range for all groups except for the BAL cells and stomach after 18 hours exposure.
- The positive control induced a statistically significant increase in the % Tail DNA in all tissues as compared to the filtered air control groups
- The presence of ‘clouds’ in the test substance groups was not consistent between test substance groups (either higher than, lower than or comparable to the % of clouds in the filtered air control group).
See 'Any other information on results incl. tables' for % tail DNA data.
CYTOTOXICITY
Local toxic effects were noted as described in the cross-referenced acute inhalation toxicity record, see section 7.2.2. At exposure concentrations of 2, 5, and 11 mg/m3, test substance-associated differences in BALF endpoints (cytology, alkaline phosphatase, lactate dehydrogenase, total protein, β-glucuronidase, and Annexin V) and microscopic changes in the liver were observed. Based on the magnitude of the differences noted in the BALF endpoints, 11 mg/m3 was considered to be the maximum tolerated dose (MTD) for local effects.
Any other information on results incl. tables
Table: % Tail DNA in BAL Cells Following Administrations of MDI (1 hour after exposure)
Treatment |
Number of Animals |
Group Mean % of Clouds |
Tail DNA (%) |
|
Mean |
S.D. (±) |
|||
Negative Control: Filtered air |
5 |
5.8 |
0.02 |
0.02 |
2.5 mg/m3 MDI |
5 |
21 |
0.03 |
0.01 |
4.9 mg/m3 MDI |
5 |
24.8 |
0.08 |
0.11 |
12 mg/m3 MDI |
5 |
20 |
0.04 |
0.05 |
Table: % Tail DNA in BAL Cells Following Administrations of MDI (18 hours after exposure)
Treatment |
Number of Animals |
Group Mean % of Clouds |
Tail DNA (%) |
|
Mean |
S.D. (±) |
|||
Negative Control: Filtered air |
5 |
19 |
2.22 |
3.33 |
2.5 mg/m3 MDI |
5 |
21.4 |
0.09 |
0.08 |
4.9 mg/m3 MDI |
5 |
32.4 |
0.43 |
0.36 |
12 mg/m3 MDI |
5 |
23.4 |
0.07 |
0.06 |
Positive Control: EMS 200 mg/kg |
5 |
41.8 |
28.82 |
4.96* |
Table: % Tail DNA in Liver Cells Following Administrations of MDI (1 hour after exposure)
Treatment |
Number of Animals |
Group Mean % of Clouds |
Tail DNA (%) |
|
Mean |
S.D. (±) |
|||
Negative Control: Filtered air |
5 |
3 |
0.15 |
0.09 |
2.5 mg/m3 MDI |
5 |
2 |
0.19 |
0.13 |
4.9 mg/m3 MDI |
5 |
4 |
0.27 |
0.37 |
12 mg/m3 MDI |
5 |
3.6 |
0.22 |
0.13 |
Table: % Tail DNA in Liver Cells Following Administrations of MDI (18 hours after exposure)
Treatment |
Number of Animals |
Group Mean % of Clouds |
Tail DNA (%) |
|
Mean |
S.D. (±) |
|||
Negative Control: Filtered air |
5 |
1 |
0.05 |
0.03 |
2.5 mg/m3 MDI |
5 |
2.8 |
0.08 |
0.08 |
4.9 mg/m3 MDI |
5 |
2 |
0.03 |
0.02 |
12 mg/m3 MDI |
5 |
2 |
0.16 |
0.14 |
Positive Control: EMS 200 mg/kg |
5 |
6.2 |
37.82 |
4.61* |
Table: % Tail DNA in Stomach Cells Following Administrations of MDI (1 hour after exposure)
Treatment |
Number of Animals |
Group Mean % of Clouds |
Tail DNA (%) |
|
Mean |
S.D. (±) |
|||
Negative Control: Filtered air |
5 |
11.6 |
10.35 |
4.39 |
2.5 mg/m3 MDI |
5 |
5.2 |
4.75 |
3.45 |
4.9 mg/m3 MDI |
5 |
3.6 |
5.27 |
4.29 |
12 mg/m3 MDI |
5 |
7 |
8.91 |
3.77 |
Table: % Tail DNA in Stomach Cells Following Administrations of MDI (1 hour after exposure)
Treatment |
Number of Animals |
Group Mean % of Clouds |
Tail DNA (%) |
|
Mean |
S.D. (±) |
|||
Negative Control: Filtered air |
5 |
15.8 |
20.18 |
5.04 |
2.5 mg/m3 MDI |
5 |
16.6 |
18.7 |
5.55 |
4.9 mg/m3 MDI |
5 |
4.4 |
16.22 |
8.71 |
12 mg/m3 MDI |
5 |
25 |
20.42 |
12.77 |
Positive Control: EMS 200 mg/kg |
5 |
55.6 |
51.35 |
4.72* |
S.D. = Standard Deviation *p ≤ 0.05 (Student's t-test); Statistically significant increase relative to the vehicle control
Applicant's summary and conclusion
- Conclusions:
- The current study is a source study used (Randazzo 2017) for read-across to the target substance MDI MT. The study data did not indicate a genotoxic potential. The genotoxic potential of the test substance to male rats was investigated in an in vivo mammalian alkaline Comet Assay/acute inhalation toxicity study according to OECD TG 489 (genotoxicity assessed by Comet Assay) under GLP conditions. This study was performed to assess if 4,4’-MDI is a genotoxic substance at the site of contact. As the site of contact tissue, bronchoalveolar lavage cells were selected; these primarily consist of alveolar macrophages, which are the primary cells responsible for the removal of inhaled aerosols from the alveoli and are commonly selected in the assessment of pulmonary genotoxicity after inhalation or instillation. In addition, the liver was included since it is the site of primary metabolism; also, it was included to investigate systemic (as opposed to local) genotoxicity. Finally, the glandular stomach was included due to possible exposure after clearance of 4,4’-MDI via the mucociliary escalator. Groups of 12 Wistar rats were exposed to actual concentrations of 2.5, 4.9 and 12mg/m3 (corresponding nominal concentrations: 2, 5, 11 mg/m3) to an aerosol-generated form of the test substance via a single nose-only inhalation exposure for 6 hours. The top concentration was defined as the maximum tolerated concentration (MTC) from a preliminary range-finding study and other supporting acute sub-lethal inhalation studies. The MTC was selected based on marked local acute toxicity as identified by biomarkers for inflammation, apoptosis/necrosis and cytotoxicity at concentrations ≥ 11.9 mg/m3. A concurrent control group received filtered air on a comparable regimen. A positive control group received ethyl methanesulfonate (EMS) via oral gavage (200 mg/kg/day) for 2 days. In preliminary studies at the performing laboratory, gavage administration of EMS resulted in a strong positive signal in all tissues examined as was determined appropriate for identifying direct acting genotoxicity. Bronchoalveolar lavage (BAL) was performed in all animals at the scheduled necropsies, and the BAL fluid (BALF) was assessed for the following endpoints: clinical chemistry (alkaline phosphatase, lactate dehydrogenase, and total protein), cell differential (cytology), and measurement of Annexin V expression and β-glucuronidase activity. The alkaline phosphatase, lactate dehydrogenase, Annexin V expression + flow cytometry, and total protein were determined to assess the cytotoxicity of the test substance. The β-glucuronidase and cell differential (in particular % polymorphonuclear leukocytes) were determined to assess the inflammatory potential of the test substance. Six rats/group were sacrificed approximately1 hour post-exposure and the other six/group approximately 18 hours post-exposure, or 2 to 4 hours after the second dose for the positive control group. At the high concentration of 12 mg/m3, test substance-associated differences in BALF endpoints (neutrophil influx, total protein, β-glucuronidase, and Annexin V) were observed. Therefore, 12 mg/m3 was confirmed to be the MTC. The test substance gave a negative (non-DNA damaging) response in this assay in the BAL cells, liver and stomach for both the 1 hour and 18 hour time points for males in % Tail DNA. It was therefore concluded that the test substance scored negative in the in vivo Comet Assay up to the maximum tolerated concentration.
- Executive summary:
Randazzo (2017) conducted a guidance study (OECD TG 489) under GLP conditions with 4,4’-MDI. As the site of contact tissue, bronchoalveolar lavage (BAL) cells were selected to be analyzed. In addition to the BAL cells, the liver and glandular stomach was included in the analysis. The liver was analyzed since it is the site of primary metabolism (and potential systemic genotoxicity), while the glandular stomach was included due to possible secondary exposure after clearance of 4,4’-MDI via the mucociliary escalator (i.e. local effects at a secondary site of contact). Groups of 12 Wistar rats were exposed to actual concentrations of 2.5, 4.9 and 12 mg/m3 for 6 hours with the maximum dose selected from pilot range-finding study and previous studies as a concentration that will induce significant local cellular damage (Hotchkiss et al., 2017). No DNA damage was noted for all three investigated tissues (BAL cells; liver; stomach) indicating that 4,4’-MDI is not genotoxic at the portal of entry at exposures of up to the maximum tolerated concentration, as indicated by local cytotoxicity.
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