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EC number: 688-501-6 | CAS number: 21700-31-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24.10. - 8.11. 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Meets national standard method (ČSN EN ISO).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Deviations:
- no
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1,1,1,2,3-pentachloropropane
- EC Number:
- 688-501-6
- Cas Number:
- 21700-31-2
- Molecular formula:
- C3H3Cl5
- IUPAC Name:
- 1,1,1,2,3-pentachloropropane
- Details on test material:
- - Name of test material (as cited in study report): 1,1,1,2,3-pentachloropropane
- Substance type: organic
- Physical state: liquid
- Analytical purity: 99.940 % (w/w)
- Impurities (identity and concentrations): chlorinated hydrocarbons - 0.048 % (w/w)
- Lot/batch No.: 2-750
- Expiration date of the lot/batch: august 2014
- Storage condition of test material: dry place at room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The stock solution of the test substance was prepared in dilution water. 20 mg of the test substance was weighed into 200 mL of the dilution water for the preliminary test and 200 mg of the test substance was weighed into 2000 mL of the dilution water for the full test. The concentrations of solutions used in the preliminary and full test were obtained by dilution of the stock solution with dilution water.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: own laboratory breeding
- Age at study initiation (mean and range, SD): max. 24 hours
- Method of breeding: aperiodic parthenogenesis
- Feeding during test: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- sum of Ca + Mg ions: cca 2.5 mmol/L
- Test temperature:
- 20 ± 2°C
- pH:
- 7.5 - 7.9
- Dissolved oxygen:
- 7.5 - 8.6 mg/L
- Nominal and measured concentrations:
- Nominal concentration:
- Preliminary test: 1, 5, 10, 50 and 100 mg/L
- Full test: 56, 62, 68, 75, 83, 91 and 100 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
Standard dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D into 1000 mL of deionized water.
Solution A: 117.6 g CaCl2·2H2O in 1 L of deionized water
Solution B: 49.3 g MgSO4·7H2O in 1 L of deionized water
Solution C: 25.9 g NaHCO3 in 1 L of deionized water
Solution D: 2.3g KCl in 1 L of deionized water
pH 7.8 ± 0.2
Sum of Ca + Mg ions: approx.2.5 mmol/L
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: natural cycle of light and dark - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 77.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 60.0 - 113.9 mg/L
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 82.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 68.3 - 215.2 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- POSITIVE CONTROL
The sensitivity of the test species and correctness of test performance was verified by testing with the reference substance, potassium dichromate: EC50 (48 hour) = 0.83 mg/L
INTERLABORATORY TEST DATA
From last interlaboratory test arranged by CSlab, s.r.o. in 2013 was obtained data for potassium dichromate 48h – EC50 generated at 13 laboratories. The following reference range of values could be calculated from those data:
EC50 (48 hour) = 0.58 – 1.34 mg/L
METHOD VALIDITY EVALUATION
The immobilisation of test organism by the reference substance (48h – EC50) obtained from reference test meets the calculated range from the interlaboratory test. The sensitivity of test species was proved.
Any other information on results incl. tables
Table No. 1: Results and conditions of preliminary and full test
Preliminary test |
|||||||||||||||
Nominal concentration |
mg/L |
100 |
50 |
10 |
5 |
1 |
control |
||||||||
Number of mobile animals |
24 hours |
0 |
10 |
10 |
0 |
10 |
10 |
||||||||
Immobilisation (%) |
24 hours |
100 |
0 |
0 |
100 |
0 |
0 |
||||||||
Number of mobile animals |
48 hours |
0 |
10 |
10 |
0 |
10 |
10 |
||||||||
Immobilisation (%) |
48 hours |
100 |
0 |
0 |
100 |
0 |
0 |
||||||||
O2 (mg/L) |
0 hour |
7.9 |
7.9 |
7.7. |
7.9 |
7.9 |
7.5 |
||||||||
O2 (mg/L) |
48 hours |
8.0 |
7.7 |
8.1 |
7.8 |
7.9 |
7.9 |
||||||||
pH |
0 hour |
7.7 |
7.6 |
7.7 |
7.7 |
7.7 |
7.8 |
||||||||
pH |
48 hours |
7.7 |
7.6 |
7.7 |
7.7 |
7.7 |
7.8 |
||||||||
Full test |
|||||||||||||||
Nominal concentration |
mg/L |
100 |
91 |
83 |
75 |
68 |
62 |
56 |
control |
||||||
Number of mobile animals |
24 hours 1stseries |
0 |
5 |
5 |
8 |
9 |
9 |
8 |
10 |
||||||
Number of mobile animals |
24 hours 2ndseries |
0 |
5 |
7 |
7 |
8 |
7 |
9 |
10 |
||||||
Number of mobile animals |
24 hours total |
0 |
10 |
12 |
15 |
17 |
16 |
17 |
20 |
||||||
Immobilisation (%) |
24 hours |
100 |
50 |
40 |
25 |
15 |
20 |
15 |
0 |
||||||
Number of mobile animals |
48 hours 1stseries |
0 |
5 |
5 |
7 |
9 |
8 |
7 |
10 |
||||||
Number of mobile animals |
48 hours 2ndseries |
0 |
4 |
6 |
4 |
5 |
6 |
8 |
10 |
||||||
Number of mobile animals |
48 hours total |
0 |
9 |
11 |
11 |
14 |
14 |
15 |
20 |
||||||
Immobilisation (%) |
48 hours |
100 |
55 |
45 |
45 |
30 |
30 |
25 |
0 |
||||||
O2 (mg/L) |
0 hour |
8.6 |
8.6 |
8.6 |
8.6 |
8.6 |
8.6 |
8.6 |
8.3 |
||||||
O2 (mg/L) |
48 hours |
8.0 |
8.0 |
7.9 |
8.2 |
8.0 |
8.2 |
7.9 |
7.9 |
||||||
pH |
0 hour |
7.8 |
7.8 |
7.8 |
7.8 |
7.8 |
7.8 |
7.8 |
7.8 |
||||||
pH |
48 hours |
7.5 |
7.5 |
7.5 |
7.5 |
7.5 |
7.5 |
7.5 |
7.5 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The determination of harmful effects of the test substance 1,1,1,2,3 - pentachloropropane exerted in short time exposure resulting in the immobilisation of daphnia. Acute toxicity is expressed as the median effective concentration EC50. Daphnia magna, Acute Immobilisation Test was performed according to the standard ČSN EN ISO 6341 (75 7751), Jakost vod – Zkouška inhibice pohyblivosti Daphnia magna Straus (Cladocera, Crustacea) – Zkouška akutní toxicity, 1997. The mentioned testing procedure is accredited as test method T18 in the testing laboratory.
The values of EC50 were calculated from equation of regression line. The NOEC and EC100 values are identified by direct observation.
Test results (nominal concentration):
EC50 (24 hour) = 82.8 mg/L (95% confidence limit: 68.3 – 215.2 mg/L)
EC50 (48 hour) = 77.2 mg/L (95% confidence limit: 60.0 – 113.9 mg/L)
NOEC (24 hour) < 56 mg/L
NOEC (48 hour) < 56 mg/L
EC100 (24 hour) = 100 mg/L
EC100 (48 hour) = 100 mg/L - Executive summary:
The determination of harmful effects of the test substance 1,1,1,2,3 - pentachloropropane exerted in short time exposure resulting in the immobilisation of daphnia. Acute toxicity is expressed as the median effective concentration EC50. Daphnia magna, Acute Immobilisation Test was performed according to the standard ČSN EN ISO 6341 (75 7751), Jakost vod – Zkouška inhibice pohyblivosti Daphnia magna Straus (Cladocera, Crustacea) – Zkouška akutní toxicity, 1997. The mentioned testing procedure is accredited as test method T18 in the testing laboratory.
The values of EC50were calculated from equation of regression line. The NOEC and EC100values are identified by direct observation.
Test results (nominal concentration):
EC50(24 hour) = 82.8 mg/L (95% confidence limit: 68.3 – 215.2 mg/L)
EC50(48 hour) = 77.2 mg/L (95% confidence limit: 60.0 – 113.9 mg/L)
NOEC (24 hour) < 56 mg/L
NOEC (48 hour) < 56 mg/L
EC100(24 hour) = 100 mg/L
EC100(48 hour) = 100 mg/L
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