Registration Dossier

Administrative data

Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993, 2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: According to a Guideline study with acceptable limitations.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Phytotoxicity studies with Lactuca sativa in soil and nutrient solution
Author:
Hulzebos EM, Adema DMM, Dirven-van Breemen, Henzen L, van Dis, WA, Herbold, HA, Hoekstra JA, Baerselman, R, van Gestel CAM
Year:
1993
Bibliographic source:
Environ. Toxicol. Chem. 12 (6): 1079-1094
Reference Type:
publication
Title:
De involoed van 50 prioritaire stoffen op de groei van Lactuca sativa
Author:
Adema DMM; Henzen L
Year:
2001
Bibliographic source:
TNO-Rapport No. 21003, TNO, Delft, Netherlands

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
Deviations:
no
Principles of method if other than guideline:
Second experiments were carried out in nurtient solutions.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Analytical monitoring were performed at test start, end of exposure and before renewal of test solution.

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
Test substance was dissolved in water.

Test organisms

Species:
Lactuca sativa
Plant group:
Dicotyledonae (dicots)

Study design

Test type:
early seedling growth toxicity test
Study type:
laboratory study
Substrate type:
other: soilo and solution (and perlite as an unreactive substrate)
Limit test:
no
Total exposure duration:
14 d
Remarks:
14 d in soil, 21 in nutrient solution

Test conditions

Test temperature:
21 +/- 4°C
pH:
7.5
Moisture:
Soil experiment: 25 to 30 % moisture content (80 % of the water-holding capacity)
Details on test conditions:
16:8 h light:dark 6500 lx fluorescent tubes

Results and discussion

Effect concentrationsopen allclose all
Species:
Lactuca sativa
Duration:
7 d
Dose descriptor:
other: EC50 soil
Effect conc.:
67 mg/kg soil dw
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: 95% C.I.: 52-86
Species:
Lactuca sativa
Duration:
14 d
Dose descriptor:
other: EC50 soil
Effect conc.:
> 100 mg/kg soil dw
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Lactuca sativa
Duration:
21 d
Dose descriptor:
other: EC50 nutrient solution
Effect conc.:
23 other: mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: 95% C.I.: (16-31)

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified