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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge nitrification inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1966
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was based on generally accepted scientific standard. Sufficient details were given.

Data source

Reference
Reference Type:
publication
Title:
Inhibition of nitrification in the activated sludge process of sewage disposal
Author:
Tomlinson TG, Boon, AG, Trotman CNA
Year:
1966
Bibliographic source:
J. Appl. Bact. 29 (2), 266-291

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
No guideline was available at the time of testing. Inhibition of nitrification process was examined.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
2 h
Remarks on exposure duration:
incubation period

Test conditions

Test temperature:
25 °C
pH:
7.6 - 7.8
Dissolved oxygen:
> 1mg/L
Salinity:
BOD 250 mg/L
50-80 mg/L ammonia
0.003 mol/L NaHCO3
0.002 mol/L (NH4)2SO4
Details on test conditions:
Pre-cleaned activated sludge in particle-free communal waste water (BOD5: 250 mg/L; NH4-N/L: 50-80 mg).

Results and discussion

Effect concentrations
Duration:
4 h
Dose descriptor:
other: EC75
Effect conc.:
12.8 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
inhibition of nitrification rate
Remarks on result:
other: duration 2-4h

Any other information on results incl. tables

The major aimof study was to develop a method.

Applicant's summary and conclusion