Registration Dossier

Administrative data

Endpoint:
fish early-life stage toxicity
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
1984
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure is similarly performed to an OECD guideline test procedure; generally accepted scientific standards were kept; water chemistry data are not reported.

Data source

Reference
Reference Type:
publication
Title:
Nucleic acid, protein content, and growth of larval fish sublethally exposed to various toxicants
Author:
Barron MG, Adelman IR
Year:
1984
Bibliographic source:
Canad. J. Fish. Aquat. Sci. 41(1), 141-150

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
Deviations:
no
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent

Sampling and analysis

Analytical monitoring:
not specified

Test organisms

Test organisms (species):
Pimephales promelas

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
32 d

Test conditions

Hardness:
soft water from lake Superior
Test temperature:
25 +/-0.2 °C
Dissolved oxygen:
>= 6.1 mg/L
Details on test conditions:
- Fluorescent lights provided 16h light per day
- Fish were fed by newly hatched brine shrimp ad libitum twice a day thereby moderate accumulation occured

Results and discussion

Effect concentrationsopen allclose all
Duration:
32 d
Dose descriptor:
NOEC
Effect conc.:
1.35 mg/L
Conc. based on:
test mat.
Duration:
32 d
Dose descriptor:
LOEC
Effect conc.:
2.57 mg/L
Conc. based on:
test mat.

Any other information on results incl. tables

NOEC for macromolecular content (nucleic acid, protein) was 2.57 mg/L (LOEC 4.20 mg/L). NOEC for growth rate was >4.2 mg/L.

Applicant's summary and conclusion