Registration Dossier

Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

Administrative data

Endpoint:
biodegradation in water: sewage treatment simulation testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1983, 1986
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: General accepted scientific principles were fulfilled.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Microbial transformation of o-cresol to dihydroxytoluenes by phenol acclimated activated sludge
Author:
Masunaga S, Urushigawa Y, Yonezawa Y
Year:
1983
Bibliographic source:
Chemosphere (12) 7/8, 1075-1082
Reference Type:
publication
Title:
Biodegradation pathway of o-cresol by heterogeneous culture
Author:
Masunaga S, Urushigawa Y, Yonezawa Y
Year:
1986
Bibliographic source:
Wat. Res. (20) 4, 477-484

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Biodegradation process by phenol acclimated activated sludge; identifying of metabolic intermediates.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, adapted
Details on inoculum:
A medium containing phenol as a carbon source was added to the activated sludge for a period of more than 1 year in a 4L laboratory unit. 950 mg/L was the concentration of microorganism in dry weight.
Duration of test (contact time):
24 h
Initial test substance concentration
Initial conc.:
200 mg/L
Based on:
test mat.

Results and discussion

Transformation products:
yes
Identity of transformation productsopen allclose all
No.:
#1
Reference
Reference substance name:
Unnamed
IUPAC name:
3-methylcatechol
Identifier:
common name
Identity:
3-methylcatechol
No.:
#2
Reference
Reference substance name:
Unnamed
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 207-827-8]
IUPAC name:
4-methylbenzene-1,3-diol
CAS number:
496-73-1
No.:
#3
Reference
Reference substance name:
Unnamed
IUPAC name:
methylhydroquinone
Identifier:
common name
Identity:
methylhydroquinone
Evaporation of parent compound:
no
Volatile metabolites:
no
Residues:
not specified

Any other information on results incl. tables

The test substance was first transformed to three dihydroxytoluenes.

Applicant's summary and conclusion