Zirconium di(acetate) oxide

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, comparable to guideline study, available as unpublished report, no restrictions, fully adequate for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Zirconium di(acetate) oxide

EC Number:

225-924-3

EC Name:

Zirconium di(acetate) oxide

Cas Number:

5153-24-2

Molecular formula:

C4 H6 O5 Zr

IUPAC Name:

zirconium di(acetate) oxide

Details on test material:

- Name of test material (as cited in study report): Zirkon-Acetat
- Lot/batch No.: 86/194
- Storage condition of test material: +4 ºC

Method

Target gene:

his

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

rat liver S9 (pretreated with Aroclor 1254)

Test concentrations with justification for top dose:

0, 20, 100, 500, 2500 and 5000 μg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: water

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene (+S9); N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) for TA 100 and TA1535 (-S9), 4-nitro-o-phenylendiamine for TA98 (-S9), 9-aminoacridine chloride monohydrate for TA1537 (-S9)

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation) (tests 1 (TA 100 and TA98) and 2 (TA1535 and TA1537)) and preincubation (test 3)

DURATION
- Preincubation period: 20 minutes at 37 °C (test 3)
- Exposure duration: 48 hours at 37 °C in the dark (test 1, 2 and 3)

NUMBER OF REPLICATIONS: 3 tests were performed; 2 standard plate incorporation assays and 1 preincubation assay. In each test 3 test plates per dose were used.

DETERMINATION OF CYTOTOXICITY
- Method: reduced his(-) background growth

Evaluation criteria:

A substance is considere positive if it fulfills the following criteria:
1) doubling of the spontaneous mutation rate (control)
2) dose-response relationship
3) reproducibility of the results

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity nor precipitates, but tested up to recommended limit concentrations

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

ADDITIONAL INFORMATION ON CYTOTOXICITY:
No bacteriotoxic effect (reduced his(-) background growth was observed.

TEST-SPECIFIC CONFOUNDING FACTORS:
Water solubility: complete solubility of the test substance in distilled water.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.