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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report date:
2004

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Zirconium di(acetate) oxide
EC Number:
225-924-3
EC Name:
Zirconium di(acetate) oxide
Cas Number:
5153-24-2
Molecular formula:
C4 H6 O5 Zr
IUPAC Name:
zirconium di(acetate) oxide
Details on test material:
- Name of test material (as cited in study report): Zirkonium-Acetat I.LSG.B.ZRO2 P.EP
- Physical state: Liquid colourless; turbid
- Analytical purity: Zirconium di(acetate)oxide: 36,6 TI
- Impurities (identity and concentrations): Water: 63.4 TI
- Batch No.: P 51

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was stirred in M4 medium for about 20 hours at 20 ± 2 °C. Undissolved test substance was removed by centrifugation (approx. 20 min. at about 17700 G).
- Differential loading: yes

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: supplied by the Institut National de Recherche Chimique Appliqueé, France, in 1978: cultured and bred continuously in the Laboratory Experimental Toxicology and Ecology of BASF SE in Ludwigshafen, Germany
- Age at study initiation: 2-24 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2,42 mmol/L
Test temperature:
19.1 - 19.6 °C
pH:
7,5 - 8,2
Dissolved oxygen:
8.4 -9.6 mg/L
Nominal and measured concentrations:
Nominal test concentrations: control, 12.5, 25, 50, 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, nominal volume, test volume: glass tubes with flat bottom, 20 ml, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels for the measurement of pH and O2 (replicates): 1
- Biomass loading rate: 0.5 animals/ml
- Feeding: none

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water (M4 medium)
- Alkalinity: 0.84 mmol
- Ca/mg ratio: 4:1
- Conductivity: 603 µS/cm
- Intervals of water quality measurement: temperature continuously during the whole test period in a separate vessel close the test vessels; pH and oxygen content after 0h in an additional replicate without daphnids and after 48h in replicate 1 of each tested
concentration.

OTHER TEST CONDITIONS
- Photoperiod: day-night rhythm = 16 :8 h
- Light intensity: ca. 1-8 µE/(m²*s) at a wave length of 400 -750 nm

EFFECT PARAMETERS MEASURED: mobility after 0, 24, 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Details on results:
EC50 (24h) = 1.21 mg/L (last control experiment: 20 Sep 2004)

Applicant's summary and conclusion