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EC number: 662-772-0 | CAS number: 25601-41-6
A study was performed to assess the ready biodegradability of the test item in an aerobic aqueous medium. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No. 301 B, “ “Ready Biodegradability; CO2Evolution Test” referenced as Method C.4-C of Commission Regulation (EC) No. 440/2008 and US EPA Fate, Transport, and Transformation Test Guidelines OPPTS 835.3110 (Paragraph (m)).
The test item, at a concentration of 10 mg Carbon/L, was exposed to inoculum consisting of sewage treatment micro-organisms and soil/ sediment filtrate with mineral medium in sealed culture vessels in the dark at approximately 21 °C for 28 d.
The degradation of the test item was assessed by the determination of carbon dioxide produced. Control solutions with inoculums and the reference item, sodium benzoate, together with a toxicity control were used for validation purposes.
The test item attained 85 % degradation after 28 d and satisfied the 10-Day window validation criterion, whereby 60 % degradation must be attained within 10 d of the degradation exceeding 10 %. The test can therefore be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301B.
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