Sulfapyridine

Additional ecotoxological information

Administrative data

Endpoint:

additional ecotoxicological information

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

2005

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: This is a publication result with a scientific method

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

GLP compliance:

no

Type of study / information:

Three 60-L instantaneous samples of municipal wastewater were collected in plastic containers lined with polyethylene bags in the morning (10-11 a.m.) for drugs analysis. The samples were kept at 4 °C in the dark during transport.

Primary cultures of rainbow trout (O. mykiss) hepatocytes were freshly prepared and plated at a density of 0.5 million/2 mL of culture medium according to a citrate/ albumin perfusion methodology (Gagne and Blaise, 2001). Fish were sexually immature at 6 months to 1 year of age and three to four livers were pooled to alleviate interindividual variability. Rainbow trout were collected from a local hatchery and acclimatized for at least 1 month in 300-L tanks filled with aerated, UV- treated, and charcoal-filtered tap water at 15 °C. They were fed with commercial trout feed three times a week.

Test material

Test material information

Constituent 1

Reference substance name:

Sulfapyridine

EC Number:

205-642-7

EC Name:

Sulfapyridine

Cas Number:

144-83-2

Molecular formula:

C11H11N3O2S

IUPAC Name:

4-amino-N-pyridin-2-ylbenzenesulfonamide

Details on test material:

no data

Results and discussion

Any other information on results incl. tables

In vitro production of oxidative metabolism in trout liver microsomes by Sulfapyridine (100µM) in the municipal effluent: 15 (NADPH oxidation rate); 5.25 (Lipid peroxidation); 0.27 (0,N,S/CH ratio)

Applicant's summary and conclusion

Conclusions:

The result show that sulfapyridine accelerated the rate of NADPH oxidation in the presence of microsomes and increased LPO in microsomal membranes.

Executive summary:

The purpose of this study was to examine the cytotoxic and oxidative effects of these products and other wastewater-related products in primary cultures of rainbow trout hepatocytes. The redox activity of various PPCPs in trout (Oncorhynchus mykiss) liver microsomes was investigated in vitro by tracking the rate of oxidation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) and the formation oflipid peroxidation (LPO) after a 60-min incubation period. In addition, primary cultures of rainbow trout hepatocytes were exposed to various drugs identified in the municipal effluent for 48 h at 15 °C. The result show thatsulfapyridineaccelerated the rate of NADPH oxidation in the presence of microsomes and increased LPO in microsomal membranes.