Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2011
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: This is a publication result with a scientific method.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2011
Report date:
2011

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: a modified DIN 38412-L34 protocol (1991).
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Sulfapyridine
EC Number:
205-642-7
EC Name:
Sulfapyridine
Cas Number:
144-83-2
Molecular formula:
C11H11N3O2S
IUPAC Name:
4-amino-N-pyridin-2-ylbenzenesulfonamide
Details on test material:
no data
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
no data

Test solutions

Vehicle:
no
Details on test solutions:
To exclude the pH-effect, test substance was prepared as a phosphate-buffered solution (0.02 M, pH 7.0, including 2 % NaCl).

Test organisms

Test organisms (species):
Vibrio fisheri
Details on inoculum:
no data

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min

Test conditions

Hardness:
no data
Test temperature:
15 °C
pH:
no data
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
no data
Details on test conditions:
The test was carried out at least twice. At least four controls (2 % NaCl solution, phosphate-buffered) were used. The test was performed at 15 °C using the thermostat. The luminescence was measured with a luminometer. The freeze-dried bacteria were rehydrated according to the test protocol; then, 500 µL aliquots of the bacteria solution were pre-incubated for 15 min at 15 °C. After the initial luminescence had been measured, 500 µL of the diluted samples were added to the bacteria. The bioluminescence was measured again after an incubation time of 30 min. The relative toxicity of the samples was expressed as a percentage inhibition compared to the controls.
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
IC50
Effect conc.:
> 50 mg/L
Conc. based on:
test mat.
Remarks on result:
other: no more data available
Details on results:
no data
Results with reference substance (positive control):
no data
Reported statistics and error estimates:
The confidence interval (a = 0.05) of the IC50 value was calculated using the R language. The dose-response curve parameters and plots were obtained using the drift package (version 0.05-92) for the R language and environment for statistical computing (www.r-pro- ject.org) as well as SigmaPlot Version 10.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The IC50 was>50 mg/l based on the inhibition of the marine bacteria Vibrio fischeri.
Executive summary:

The 30-min standard bioluminescence inhibition assay was carried out according to a modified DIN 38412-L34 protocol (1991). The IC50 was>50 mg/l based on the inhibition of the marine bacteria Vibrio fischeri.