2,3-epoxypropyl phenyl ether

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1984

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented standard NTP study

Data source

Reference

Reference Type:

publication

Title:

Comparative mutagenicity of aliphatic epoxides in Salmonella

Author:

Canter, D.A., Zeiger, E., Haworth, S., Lawlor, T., Mortelmans, K., and Speck, W.

Year:

1986

Bibliographic source:

Mutation Res. Vol. 172 (1986) 105-138

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Principles of method if other than guideline:

four strains only, but data for all strains are positive

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Automatically generated during migration to IUCLID 6, no data available

IUPAC Name:

Automatically generated during migration to IUCLID 6, no data available

Details on test material:

CAS: 122-60-1

Method

Target gene:

HIs operon

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA100, TA1535, TA97, TA98

Metabolic activation:

with and without

Metabolic activation system:

Hamster and Rat S9

Test concentrations with justification for top dose:

0, 10, 33, 100, 333, 1000

Controls

Untreated negative controls:

yes

Positive control substance:

other: TA98, 2-nitrofluorene or alternatively, TA98 and TA1538, 4-nitro-o-phenylenediamine TA100 and TA1535, sodium azide TA97 and TA1537, 9-aminoacridine, 2-aminoanthracene

Details on test system and experimental conditions:

In the standard protocol (preincubation) for conducting the Ames assay, a test tube containing a suspension of one strain of Salmonella typhimurium (or E. coli) plus S9 mix or plain buffer without S9, is incubated for 20 minutes at 37º C with the test chemical. Control cultures, with all the same ingredients except the test chemical, are also incubated. In addition, positive control cultures are prepared; these contain the particular bacterial tester strain under investigation, the various culture ingredients, and a known potent mutagen*. After 20 minutes, agar is added to the cultures and the contents of the tubes are thoroughly mixed and poured onto the surface of Petri dishes containing standard bacterial culture medium. The plates are incubated, and bacterial colonies that do not require an excess of supplemental histidine appear and grow. These colonies are comprised of bacteria that have undergone reverse mutation to restore function of the histidine-manufacturing gene. The number of colonies is usually counted after 2 days.

Evaluation criteria:

Several doses (usually at least 5) of each test chemical and multiple strains of bacteria are used in each experiment. In addition, cultures are set up with and without added liver S9 enzymes at varying concentrations. Therefore, a variety of culture conditions are employed to maximize the opportunity to detect a mutagenic chemical. In analyzing the data, the pattern and the strength of the mutant response are taken into account in determining the mutagenicity of a chemical. All observed responses are verified in repeat tests. If no increase in mutant colonies is seen after testing several strains under several different culture conditions, the test chemical is considered to be nonmutagenic in the Ames test.

Results and discussion

Test results

Species / strain:

S. typhimurium, other: all strains

Metabolic activation:

with and without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
positive

The test substance is positive in vitro.