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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

No inhibition of respiration of activated sludge was observed up to 100 mg/L the highest tested concentration in an OECD 209 compliant study.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
100 mg/L

Additional information

The purpose of this study was to determine the effect of the test item on bacteria according to the OECD guideline 209 "Activated Sludge, Respiration Inhibition Test" and the OECD principles of Good Laboratory Practices.

The effect of the test item on bacteria is assessed by measuring the micro-organisms respiration rate on a sample of activated sludge during an incubation period of 3 hours.


The EC50calculations of respiration inhibition rate of reference and test substances are presented hereafter:

Test item: EC50- 3h > 100 mg/L

3,5-Dichlorophenol = 9.43 mg/L