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EC number: 235-120-4 | CAS number: 12070-08-5
No effects were observed on the body weights and the lymph node weights of the animals tested with titanium carbide. No other signs of toxicity were seen in any of the animals.
Table 1: Mean weight of lymph nodes.
Mean of test group
12.5% TiC in vehicle
25% TiC in vehicle
50% TiC in vehicle
In this dermal sensitization study with titanium carbide in acetone/olive oil 4:1 (v/v), young adult female CBA/CaOlaHsD mice were tested using the Local Lymph Node Assay (LLNA). 25 µl of the test substance in solvent were applied on the entire dorsal ear surface of each animal. The following concentrations were used: 12.5, 25 and 50%. Acetone/olive oil 4:1 was used as negative control. Phenylenediamine was tested as a positive control in a prior assay. The application was repeated daily for three consecutive days. The doses applied were selected based on a pre-screening assay. Five days after the 1st application 250 µL 20 µCi 3H-methyl thymidine was injected intravenously to all mice (diluted concentration: 80 µCi/mL). All animals were sacrificed five hours after the aforementioned injection. The draining ear lymph nodes were excised into PBS and single cell suspension of lymphocyte was isolated. Cells were precipitated with 5% trichloroacetic acid at 4 °C for 18 hours.The radioactivity was measured in a ß-counter.
Stimulation indices (ratio of 3H-methyl thymidine incorporation into lymph node of test animals relative to that for control animals)≥ 3 were considered a positive response. The results revealed the following SI: 1.8, 1.7 and 1.9 for the concentrations of 12.5, 25 and 50%, respectively. No signs of toxicity were detected in any of the treated animals. In this study, Titanimum carbide is not a dermal sensitizer.
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