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Diss Factsheets
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EC number: 485-110-0 | CAS number: 53535-81-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-11-23
- Reliability:
- 1 (reliable without restriction)
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 88/303/EC
- Deviations:
- no
- Principles of method if other than guideline:
- no other principles
- GLP compliance:
- yes (incl. QA statement)
Test material
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
NA
Sampling and analysis
- Analytical monitoring:
- not required
- Details on sampling:
- NA
Test solutions
- Vehicle:
- no
- Details on test solutions:
- In the main test an aqueous stock solution of Lithium-tert-amoxide (LTA) was prepared with a test item concentration of 200 mg/L, and during the performance of the test the test solutions were mixed by mechanical stirring to ensure a good dispersion.
Because the test item reacts violently with water a complementary experiment was carried out to repeat and confirm the 0-7 day results of the main test.
During the performance of the complementary experiment defined amounts of test item were directly weighed into the large glass bottles with valve to reach the planned nominal concentrations (2 mg/L). The nominal test item concentrations were prepared by mechanical dispersion (intensive stirring). These test solutions were freshly prepared at the beginning of the experiment, in the testing laboratory.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in isotonic saline solution and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 4 g dry material per litre were mixed with test water (see below) and then aerated until use. Before use the sludge was filtered through cotton wool.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- NA
Test conditions
- Hardness:
- NA
- Test temperature:
- 19.4 – 20.3 °C (during the incubation) and
19.2 – 22.0 °C (during oxygen measurement) - pH:
- The pH of the activated sludge inoculum was determined to be pH 7.43.
- Dissolved oxygen:
- The concentration of dissolved oxygen did not drop below 2.5 mg O2/L during the incubation period, and just before the measurements of the respiration rates the oxygen concentrations were at least 7.3 mg O2/L.
- Salinity:
- NA
- Nominal and measured concentrations:
- nominal: 10, 31, 100, 313 and 1000 mg/L
no concentrations measured - Details on test conditions:
- aerobic incubation
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 185.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% conf. limit 156.8–219.9
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The NOEC for respiration rates was not based on the results of a statistical analysis, but biologically justified. The respiration rates were inhibited and influenced dose dependently in the whole concentration range, however the observed slight inhibitions (4.7 % and 6.5 %) at the concentration levels of 10 and 31 mg/L were evaluated as reflecting the biological variability in the test. At the concentration of 100 mg/L the calculated respiration rate (0.470) was in the historical control data range (0.490 - 0.089), and the acceptable range of controls of +/- 15 %. Thus, the observed inhibition at 100 mg/L of < 15% can be considered as a biological variability of the test system.
- Results with reference substance (positive control):
- The 3-hour EC50 of the reference item 3,5-Dichlorophenol for the used activated sludge batch was determined to be 8.20 mg/L and thus the EC50 was in the acceptable range of 5 to 30 mg/L.
- Reported statistics and error estimates:
- NA
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (3h) = 185.7 mg/l
- Executive summary:
In comparison to the inoculum controls the respiration rate of the activated sludge was inhibited dose-dependently in the whole examined concentration range, displaying inhibition rates from 4.7 up to 98.1 %.
NOEC (3h) = 100 mg/l. EC50 (3h) = 185.7 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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