N-(2-nitrophenyl)phosphoric triamide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005-02-01 - 2005-07-13

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

OECD 202 (2004)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

200 mg CaCO3/L

Test temperature:

20.5 - 20.1 degree C

pH:

8.02

Dissolved oxygen:

8.66 mg/l

Nominal and measured concentrations:

100 mg/l

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

No effects of N-(2-Nitrophenyl)phosphoric triamide on immobilisation of daphnids were determined. The NOEC after 48 h was 100 mg/l.

Executive summary:

In a 48-h acute toxicity study according to OECD guideline 202, water fleas (Daphia magna) were exposedunder static conditions

to analytically measured test concentrations of 100 mg/LN-(2-Nitrophenyl)phosphoric triamide. The aim of this acute toxicity test was to assess possible effects of the test item on daphnids. Young daphnids, aged less than 24 hours at the start of the test, were exposed to the test item for a period of 48 hours. They were kept in a sample of defined water (ISO 6341) to which the test item has been added. Immobilisation was recorded at 24 and 48 hours after the test start and compared with control values. To determine the concentration for the test, a range-finding test (under non-GLP conditions) was performed. Because no immobilisation of Daphnia occurred during the range-finder up to the highest test concentration, the test was performed as a limit test at the highest possible test concentration of lOOmg/L.

No effects of N-(2-Nitrophenyl)phosphoric triamide on immobilisation of daphnids were determined. The NOEC after 48 h was 100 mg/l.

Description of key information

OECD 202 guideline study, GLP: NOEC: 100 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

100 mg/L

Additional information

In a 48-h acute toxicity study according to OECD guideline 202, water fleas (Daphia magna) were exposedunder static conditions

to analytically measured test concentrations of 100 mg/LN-(2-Nitrophenyl)phosphoric triamide.

No effects of N-(2-Nitrophenyl)phosphoric triamide on immobilisation of daphnids were determined. The NOEC after 48 h was 100 mg/l.