2-(2-hexyloxyethoxy)ethanol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Althought the study methodology followed OECD TG 203, EPA OTS 797.1400 and ASTM guidelines, it is unclear whether the study was conducted as per Good Laboratory Practices (GLP) and the report contains no information on analytical measurements of the tested doses.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

EPA OTS 797.1400 (Fish Acute Toxicity Test)

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

other: Annual Book of ASTM Standards, Water and Environmental Technology, Vol. 11.05, (1995)

Deviations:

not specified

Principles of method if other than guideline:

not applicable

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): Hexyl Carbitol Solvent
- Physical state: liquid

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Dechlorinated Charleston municipal water was used in tests as dilution water and in experimental controls and the same water was used for maintaining the in-house fathead minnow stock. The incoming water was treated with activated carbon in a two-gallon filter yielding a chlorine-free dilution water with a pH very close to 7.0. The carbon beds were changed as needed to maintain water quality.

Test organisms (species):

Pimephales sp.

Details on test organisms:

TEST ORGANISM
- Common name: fathead minnows
- Strain: no information available
- Source: in-house stock (UCC laboratory culturing program)
- Age at study initiation (mean and range, SD): 21-60 days
- Length at study initiation (length definition, mean, range and SD): average fish length ranged from 10-15 mm
- Weight at study initiation (mean and range, SD): average wet weight/fish ranged from 10-25 grams
- Other: organism loading ranged from 0.13-0.33 g wet-weight fish/dose

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

40 to 60 mg/L as CaCO3 (measured)

Test temperature:

no information available

pH:

close to 7.0

Dissolved oxygen:

no information available

Nominal and measured concentrations:

- Nominal concentrations: no information available.

Details on test conditions:

A minimum of 5 concentrations of each test substance were utilized, with each 1.5 liter volume containing 20 fish fry. Fish survival and physical response, as well as temperature, pH and dissolved oxygen levels, were monitored daily during the test period of 96 hours.

Reference substance (positive control):

not required

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL -180-210 mg/L

Reported statistics and error estimates:

The concentration of test product which affected 50% of the test population was calculated for the 96-hour exposure period by the Spearman-Karber estimator. The 95% confidence intervals were also provided by this program.

Sublethal observations / clinical signs:

ADDITIONAL REPORTED EFFECT CONCENTRATIONS

96-h NOEC = 95 mg/L

Description of key information

DGHE is with high probability acutely not harmful to fish.

The 96-h LC50 value is 200 mg/L in freshwater fish (Pimephales sp.)

Additional information

The short-term toxicity to freshwater fish was determined in a study according to OECD TG 203. In this study, groups of 20 Pimephales sp. were exposed for 96 hours to five concentrations of test substance under static conditions. No analytical verification of the test material performed. The 96-hour LC50 of the test material was calculated to be 200 mg/L (95% fiducial limits ranging between 180-210 mg/L).