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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Description of key information

2 -Methylimidazole was not a skin sensitiser in a local lymph node assay in the mouse.

Key value for chemical safety assessment

Skin sensitisation

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not sensitising)
Additional information:

The skin sensitising properties of 2-methylimidazole were determined in a GLP study according to OECD guideline 429 (Local Lymph Node Assay (LLNA)) and EU method B.42 (BASF SE, 2012). For this purpose, three groups of 5 female mice each were treated with increasing concentrations of 2-methylimidazole (10%, 25% and 50% in vehicle, respectively) applied on the dorsum of both ears (25 µL per ear) for three consecutive days. Three days after the last treatment, all animals received an intravenous injection of 3H-methyl Thymidine (3HTdR). Five hours later, the 3HTdR incorporation in the draining auricular lymph nodes was determined. The results were compared with those of a control group which was treated with the vehicle (ethanol:sterile water, 7:3 v/v). A positive control group treated with α-hexyl cinnamaldehyde (up to 25% HCA solution in acetone:olive oil, 4:1 v/v) was included in the report as a reliability check.

No signs of systemic toxicity or local skin irritation were observed.

Stimulation indices (SIs) of 1.02, 1.07 and 1.14 were calculated in response to a 10%, 25% and 50% 2-methylimidazole concentration, respectively. Since the SIs were lower than 3 at all concentrations the results indicted that 2-methylimidazole should not be considered a skin sensitiser (a SI of 3 is the limiting value required for classification as a skin sensitiser).

Respiratory sensitisation

Endpoint conclusion
Endpoint conclusion:
no study available

Justification for classification or non-classification

Based on the results of the skin sensitisation study, 2-methylimidazole does not need to be classified according to the Regulation (EC) No. 1272/2008.