Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to guideline and/or standard method but there were no data on GLPs.
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Methods for Measuring the Acute Toxicity of Effluents to Freshwater and Marine Organisms, EPA/600/4-85/013 March 1985.
Principles of method if other than guideline:
Not applicable
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Test material: Propyl CELLOSOLVE
Source: Union Carbide Corporation
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
No data

Test solutions

Vehicle:
not specified
Details on test solutions:
Dechlorinated Charleston municipal water was used in tests as dilution water and in experimental controls. This same water was used for
maintaining fathead minnow population. The incoming water was treated with activated carbon in a series of two 8-10 gallon filters yielding a
chlorine-free dilution water with a pH very close to 7.0. Total hardness of this water measured 40 to 60 mg/L as CaCO3. The carbon beds were changed as needed to maintain water quality.

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
The test fish, fathead minnows, were obtained from Union Carbide's laboratory culturing program. Size of the minnows ranged from 1.5-3 cm. Any chemical treatment required to control or prevent disease was stopped at least five days prior to using the fish in tests.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
None

Test conditions

Hardness:
No data
Test temperature:
No data
pH:
No data
Dissolved oxygen:
No data
Salinity:
No data
Nominal and measured concentrations:
No data
Details on test conditions:
The definitive tests were conducted by EPA-ASTM procedures using 10 fathead minnows per test concentration in a total volume of 0.75 liters.
Temperature, fish survival, pH and dissolved oxygen levels were monitored during the test period of 96 hours. Minimal aeration was supplied when the dissolved oxygen was below about 4 mg/L.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 5 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No additional data
Results with reference substance (positive control):
No data
Reported statistics and error estimates:
None

Any other information on results incl. tables

None

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The 96h acute LC50 in fish is >5000 mg/L.
Executive summary:

96 hour Static definitive tests were conducted by EPA-ASTM procedures using 10 fathead minnows (Pimephales promelas) per test concentration in a total volume of 0.75 liter. Temperature, fish survival, pH and dissolved oxygen levels were monitored during the test period of 96 hours. Minimal aeration was supplied when the dissolved oxygen was below about 4 mg/L.

The 96h acute LC50 in fish was found to be >5000 mg/L.