1,3-Benzenedicarboxylic acid, 5,5'-[1,2-ethanediylbis[thio-1,3,4-thiadiazole-5,2-diyl-2,1-diazenediyl[5-amino-3-(1,1-dimethylethyl)-1H-pyrazole-4,1-diyl]]]bis-, potassium salt (1:4)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

04.05.11 to 08.06.11

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Report on substance meets the criteria for classification as reliable without restriction according to Klimisch et al (1997)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

tetrapotassium 5-{5-amino-4-[(1E)-2-(5-{[2-({5-[(1E)-2-[5-amino-3-tert-butyl-1-(3,5-dicarboxylatophenyl)-1H-pyrazol-4-yl]diazen-1-yl]-1,3,4-thiadiazol-2-yl}sulfanyl)ethyl]sulfanyl}-1,3,4-thiadiazol-2-yl)diazen-1-yl]-3-tert-butyl-1H-pyrazol-1-yl}benzene-1,3-dicarboxylate

EC Number:

700-616-6

Cas Number:

849608-59-9

Molecular formula:

C36H32K4N14O8S4

IUPAC Name:

tetrapotassium 5-{5-amino-4-[(1E)-2-(5-{[2-({5-[(1E)-2-[5-amino-3-tert-butyl-1-(3,5-dicarboxylatophenyl)-1H-pyrazol-4-yl]diazen-1-yl]-1,3,4-thiadiazol-2-yl}sulfanyl)ethyl]sulfanyl}-1,3,4-thiadiazol-2-yl)diazen-1-yl]-3-tert-butyl-1H-pyrazol-1-yl}benzene-1,3-dicarboxylate

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, adapted

Details on inoculum:

- Source of inoculum/activated sludge: Totnes Sewage Treatment Works, Totnes, Devon, UK
- Storage conditions: Room temperature, aerated, maintained at pH 7.0 +/- 1 degree centigrade.
- Preparation of inoculum for exposure: Centrifuged, washed and resuspended in mineral medium
- Concentration of sludge: 30 mg/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 22 degrees centigrade +/- one degree
- pH: 6.7-7.5
- Continuous darkness: yes

TEST SYSTEM
- Measuring equipment: Oxitop respirometer
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used:CO2 absorbed by KOH solution in a seal cup in neck of bottle

SAMPLING
- Sample storage before analysis: ambient temperature

Reference substance

Reference substance:

benzoic acid, sodium salt

Remarks:

100 mg/L

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

5 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

10 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

15 d

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

20 d

% Degradation 5

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

Sodium benzoate attained a maximum mean level of biodegradation (based on BOD:ThOD ratio) of 67%. >60% degradation was acheived in the 10 day window.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

It is concluded that in this study the test substance failed to meet the criteria for ready biodegradability.