Registration Dossier
Registration Dossier
Diss Factsheets
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EC number: 202-213-6 | CAS number: 93-04-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- experimental data of target chemical and read across substances
- Justification for type of information:
- Experimental test result performed by following guidelines similar to standard OECD 203 guidleines.
- Reason / purpose for cross-reference:
- other: Target chemical
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- WoE3: The test concentrations selected for the study were 0, 3.125 mg/L, 6.25 mg/L,12.5 mg/L, 25 mg/L, 50 mg/L.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- WoE2:
TEST ORGANISM
- Common name:Zebra Fish
WoE3:
TEST ORGANISM
- Common name:Zebra Fish
- Length at study initiation (length definition, mean, range and SD): average 1.86 cm
- Weight at study initiation (mean and range, SD): average 0.084 g
- Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
- Duration of housing: 15 days
- Photoperiod: 16 hour light and 8 hour darkness
- Diet : Standard brand feed, once daily
- Acclimatization: seven days
- Feeding frequency: Once in a day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- WoE3: 152.5 mg of CaCO3
- Test temperature:
- WoE3: 23.8 °C
- pH:
- WoE3: 7.1
- Dissolved oxygen:
- WoE3: 7 mg/l
- Nominal and measured concentrations:
- WoE3:Nominal concentration 0, 3.125 mg/L, 6.25 mg/L,12.5 mg/L, 25 mg/L, 50 mg/L.
- Details on test conditions:
- WoE3:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastiic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Photoperiod: 16 hour light and 8 hour darkness
- Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 21.4 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 28 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on various data obtained from different sources, the median lethal concentration LC50 of test chemcial to fresh water fish was in the range of 21.4 to 50mg/L.
- Executive summary:
Data available for target chemical and structurally & functionally similar read across analogues, has been reviewed to determine the effect caused on fresh water fishes. The studies are summarised below:
In a prediction done using the OECD QSAR toolbox version 3.4, the short term toxicity on fish was predicted for test chemical for 96 hr. EC50 value was estimated to be 21.4 mg/l on Danio rerio (Zebra fish) for 96 h duration.
Another short term toxicity to fish study was conducted for 96 hrs for assessing the effect of test chemical on Zebra fish (Danio rerio). The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.084 g and average length of 1.86 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.0 mg/l, pH 7.4, water temperature 23.8°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. Nominal test chemical conc. used for the study were 0, 3.125 mg/L, 6.25 mg/L,12.5 mg/L, 25 mg/L, 50 mg/L, respectively. Total 48 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23.8°C, pH 7.1, hardness of water 152.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. The fishes were moving slowly in the test chemical conc. as compared to the control. No mortalities were observed in the control vessel. On the basis of effect of test chemical on the mortality of test organism, the median lethal concentration (LC50 (96 h)) value was determined to be 50 mg/l.
For the test chemical, short term fish toxicity study was conducted for 96 hrs for assessing the effect of test chemical. Study was performed in a static fresh water system. On the basis of effect of test chemical on the mortality of the test organism fish, the 96 hrs LC50 value was determined to be 28 mg/l.
On the basis of the predicted data & various experimental studies of the test chemicals and applying the weight of evidence approach, the LC50 value of the test chemical on test organism fish can be expected to be in the range of 21.4 to 50 mg/L, respectively. Thus, based on the LC50 value, test chemical can be considered as toxic to fish and considered to be classified in ‘aquatic chronic category 3’ as per CLP classification criteria.
Reference
Description of key information
Based on various data obtained from different sources, the median lethal concentration LC50 of test chemcial to fresh water fish was in the range of 21.4 to 50mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 50 mg/L
Additional information
Data available for target chemical and structurally & functionally similar read across analogues, has been reviewed to determine the effect caused on fresh water fishes. The studies are summarised below:
In a prediction done using the OECD QSAR toolbox version 3.4, the short term toxicity on fish was predicted for test chemical for 96 hr. EC50 value was estimated to be 21.4 mg/l on Danio rerio (Zebra fish) for 96 h duration.
Another short term toxicity to fish study was conducted for 96 hrs for assessing the effect of test chemical on Zebra fish (Danio rerio). The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.084 g and average length of 1.86 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.0 mg/l, pH 7.4, water temperature 23.8°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. Nominal test chemical conc. used for the study were 0, 3.125 mg/L, 6.25 mg/L,12.5 mg/L, 25 mg/L, 50 mg/L, respectively. Total 48 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23.8°C, pH 7.1, hardness of water 152.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. The fishes were moving slowly in the test chemical conc. as compared to the control. No mortalities were observed in the control vessel. On the basis of effect of test chemical on the mortality of test organism, the median lethal concentration (LC50 (96 h)) value was determined to be 50 mg/l.
For the test chemical, short term fish toxicity study was conducted for 96 hrs for assessing the effect of test chemical. Study was performed in a static fresh water system. On the basis of effect of test chemical on the mortality of the test organism fish, the 96 hrs LC50 value was determined to be 28 mg/l.
On the basis of the predicted data & various experimental studies of the test chemicals and applying the weight of evidence approach, the LC50 value of the test chemical on test organism fish can be expected to be in the range of 21.4 to 50 mg/L, respectively. Thus, based on the LC50 value, test chemical can be considered as toxic to fish and considered to be classified in ‘aquatic chronic category 3’ as per CLP classification criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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