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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
During the final test samples for analysis were taken from 0.32, 1.0 and 3.2 mg/I. Also samples were taken from the control. The method of analysis isdescribed in the appended Analytical Report.
Sampling:
Frequency: at 24 hours and 48 hours
Volume: 10 ml from the approximate centre of the test vessel
Storage: The samples were stored in a freezer until analysis

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Start of each batch: With new-born animals, Le. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture
vessel.
Maximum age of the cultures: 4 weeks
Renewal of the cultures: After 7 days of cultivation half of the medium twice a week.
Temperature of medium: 18-22°C, constant within ± 1°C
Feeding: Daily, a suspension of fresh water algae.


Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
19.7-20.5°C
pH:
7.7-7.9
Dissolved oxygen:
8.0-9.0 mg O2/L
Nominal and measured concentrations:
nominal 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
3.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 2.7-4.8 mg/l
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 1.2-1.9 mg/l
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.56 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Analysis of the samples taken showed that the measured concentrations were in agreement with nominal (98 -107%).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48h-EC50 was 1.5 mg/l with a 95% confidence intervall between 1.2 and 1.9 mg/l.