Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Qualifier:
according to
Guideline:
EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
The sludge was kept under continuous aeration until further treatment. The concentration of suspended solids was 3.9 g/l in the concentrated sludge
(information obtained from the municipal sewage treatment plant). Before use, the sludge was allowed to settle (30-90 minutes) and the liquid
decanted for use as inoculum at the amount of 10 mI/I of mineral medium.
Duration of test (contact time):
29 d
Initial test substance concentration
Initial conc.:
14.5 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
Test duration
28 days (last CO2-measurement on the 29th day). During the test period aeration and stirring took place.
Test vessels
2 litre all-glass brown coloured bottles.
Milli-RO / Milli-Q water
Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ionexchange cartridges (Milli-Q) (Millipore
Corp., Bedford, Mass., USA).
Sampling
0, 2, 5, 7, 9, 14, 19, 23, 27 and 29 days
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (CO2 evolution)
Value:
0
Sampling time:
0 d
Remarks on result:
other: mean of bottle A and B
Parameter:
% degradation (CO2 evolution)
Value:
1
Sampling time:
5 d
Remarks on result:
other: mean of bottle A and B
Parameter:
% degradation (CO2 evolution)
Value:
1
Sampling time:
14 d
Remarks on result:
other: mean of bottle A and B
Parameter:
% degradation (CO2 evolution)
Value:
1
Sampling time:
23 d
Remarks on result:
other: mean of bottle A and B
Parameter:
% degradation (CO2 evolution)
Value:
1
Sampling time:
29 d
Remarks on result:
other: mean of bottle A and B

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
MMC was not readily biodegradable under the conditions of the modified Sturm test presently performed.