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EC number: 252-021-1 | CAS number: 34432-92-3
The results obtained in most experiments did not show substantial (biologically significant) increases in the number of revertants versus solvent controls (Rt/ Rc < 2), and no experiment gave evidence of a rising trend in the number of revertants with increasing dose.
In some cases, experiments with increased values of revertants occurred, exclusively with metabolic activation. It concerns the following experiments:
- TA 100 +MAII, trend to 500 µg per plate Rt/Rc max =1.5;
- TA 1535 +MAI trend to 1500 µg per plate Rt/Rc max =1.7;
- TA 98 +MAI trend to 500 µg per plate ,max Rt/Rc 1.9 and experiment +MAII, max Rt/Rc =1.6;
- E. coli + MAII, without any trend, max. Rt/Rc =1.5; increase caused rather by lower solvent control value.
In case of TA 100, TA 1535 and E.coli the results were neither confirmed by the parallel experiments nor Rt/Rt reached 2. In case of TA 98, increased values of revertants were observed in both experiments, while dose-response relationship was observed in the first experiment only. It could be caused relatively small enhancement of number of revertants and large variability of the biological system. Anyway, such increasing is not common in negative experiments.
Test substance Solvent Yellow 124 was assayed for the mutagenicity by the Bacterial Reverse Mutation Test.
The performed test was based on EU method B.13/14 Mutagenicity – Reverse mutation test using bacteria, which is analogous to the OECD Test Guideline No. 471.
Four indicator Salmonella typhimurium strains TA 98, TA 100, TA 1535, TA 1537 and one indicator Escherichia coli WP2 uvrA strain were used. The test substance was dissolved in dimethylsulfoxide and assayed in doses of 5-5000 µg, which were applied to plates in volume of 0.1 mL.
Experiments were performed without as well as with metabolic activation with a supernatant of rat liver and a mixture of cofactors.
In the arrangement given above, the test substance Solvent Yellow 124 was nonmutagenic for all the Salmonella typhimurium as well as Escherichia coli strains in experiments without metabolic activation. It was nonmutagenic also for Salmonella typhimurium TA 100, TA 1535 and TA 1537 and for Escherichia coli WP2 uvrA with metabolic activation.
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