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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations.

Key value for chemical safety assessment

Additional information

The toxicity of benzylamine (CAS 100-46-9) to microorganisms was investigated in a 40-h growth inhibition test with Tetrahymena pyriformis (Schultz, 1997) conducted to generally accepted scientific principles. Based on nominal test concentrations, a 40-h IC50 of 186 mg/L was determined; the study is acceptable for assessment and is selected as key data.


The key study is supported by two read-across studies on the structurally similar substance benzyldimethylamine (CAS 103-83-3). With this substance, a respiration inhibition test with activated sludge according to the OECD guideline 209 was conducted. As the EC20 after 30 min was determined at the highest test concentration of 575 mg/L, the EC50 is concluded to be beyond this concentration (BASF AG, 1987). However, the activated sludge in this study was taken from an industrial source instead of a domestic sewage treatment plant. In the second study with benzyldimethylamine (CAS 103-83-3), a growth inhibition test with Pseudomonas putida (according to Bringmann-Kuehn), an EC50 value was determined at 750 mg/l after 17 h.


In addition a supporting guideline study (OECD 209) was conducted with the structurally similar substance methylbenzylamine (CAS 3886-69-9). An EC20 value was determined at 550 mg/L after 30-min, the EC50 was beyond the highest test concentration of 1000 mg/L (BASF AG, 1998). All validity criteria were fulfilled; the activated sludge was taken from a domestic source.

Based on the available data, toxic effects on microorganisms are not to be expected for benzylamine.