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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Description of key information

* OECD 207 (same results for test mat. 'Distillates (Fischer-Tropsch), C8-26 - branched and linear' and Distillates (Fischer-Tropsch), heavy, C18-50 - branched, cyclic and linear'):
- LC50 (14-d): >1000 mg/kg dw test mat. (nominal) based on: mortality
- NOEC (14-d): ≥1000 mg/kg dw test mat. (nominal) based on: mortality;
* OECD Test Guideline 222 (test mat. 'Distillates (Fischer-Tropsch), C8-26 - branched and linear'):
NOEC (28-d): ≥1000 mg/kg dw test mat. (nominal) based on: survival and reproduction;
* OECD Test Guideline 222 (test mat. 'Distillates (Fischer-Tropsch), heavy, C18-50 - branched, cyclic and linear'):
- LC50 (4-w): >1000 mg/kg dw test mat. (nominal) based on: growth, reproduction and mortality
- NOEC (4-w): ≥1000 mg/kg dw test mat. (nominal) based on: growth, reproduction and mortality.
Short and long-term soil macroorganism toxicity data are available for two closely related substances in the relevant carbon number range for members of the Category "Hydrocarbons, C14 -C20, aliphatics (<2% aromatics)”. In the absence of adverse effects in any of these studies, based on weight of evidence, it can be concluded that the NOEC for the registered substance is ≥1000 mg/kg dw soil.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
1 000 mg/kg soil dw
Long-term EC10, LC10 or NOEC for soil macroorganisms:
1 000 mg/kg soil dw

Additional information

An acute earthworm toxicity study is available for the closely related substance “Distillates (Fischer-Tropsch), C8-26 - branched and linear”, covering the carbon range, C8-C26 (Priestly, 2010b). The test was conducted in accordance with OECD 207 and GLP, however no analytical monitoring of the test substance in the exposure medium was carried out. Earthworms (Eisenia foetida) were exposed to a single concentration of the test material at a nominal loading rate of 1000 mg/kg of soil (dry weight) for a period of 14 days. There were no significant mortalities or sub-lethal effects observed during the 14-day exposure period. The 14-d acute LC50for earthworms was therefore determined to be greater than 1000 mg/kg dw.

An identical short-term earthworm toxicity study is also available for “Distillates (Fischer-Tropsch), heavy, C18-50 - branched, cyclic and linear” (Priestly, 2010b). The 14-d acute LC50was determined to be greater than 1000 mg/kg dw as well.

In addition, an earthworm reproduction study, conducted in accordance with OECD 222 and GLP has also been performed with “Distillates (Fischer-Tropsch), C8-26 - branched and linear” (Schmidt, 2011a). No analytical monitoring was carried out. Adult earthworms were exposed in treated soils for a period of four weeks, at test concentrations of 10, 32, 100, 320, and 1000 mg per kg dry soil. Additionally, a control and a reference item treatment were tested in parallel. After this period, the adults were removed from the test vessels and the survival and growth rate were determined. The cocoons and juvenile earthworms remained in the vessels for additional four weeks. The reproduction rate was determined by counting the number of offspring hatched from the cocoons after this additional test period of four weeks. The NOEC of this study based on survival, growth and reproduction parameters was at least 1000 mg/kg dw. A LOEC could not be established due to the absence of adverse effects at the highest dose level.

A long-term toxicity study withE. foetida has been conducted with Distillates (Fischer-Tropsch), heavy, C18-50 - branched, cyclic and linear (Schmidt, 2011b) in accordance with GLP and OECD 222 as well. Adult test organisms were exposed to 10, 32, 100, 320 and 1000 mg/kg dw soil for 4 weeks and then removed, while the juvenile earthworms remained in the vessels for an additional 4 weeks. Adult mortality and mean change in body weight were recorded, as well as mean reproduction rate of juveniles. Based on these parameters an LC50and a LOEC could not be determined and a NOEC of at least 1000 mg/kg dw has been determined.

Conclusion

Short and long-term soil macroorganism toxicity data are available for two closely related substances in the relevant carbon number range for members of the Category "Hydrocarbons, C14 -C20, aliphatics (<2% aromatics)”. In the absence of adverse effects in any of these studies, based on weight of evidence, it can be concluded that the NOEC for the registered substance is ≥1000 mg/kg dw soil.