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EC number: 257-036-7 | CAS number: 51181-50-1
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
48h EC50 100.9 mg/l EDTA-FeNa for Daphnia magna, corresponding to 97 mg/L HEDTA-FeNa
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 97 mg/L
Additional information
Four studies report on the toxicity of the test substance to Daphnia magna.
1. The toxicity of FeNaEDTA on Daphnia magna was tested in a 48 h static immobilisation test (Borrmann 2007). Test concentrations were measured and were within the range of 80% of the nominal concentration. The EC50 (mobility) was 100.9 mg/L (nominal) and the NOEC was 30 mg/L (reliability 1, Key study).
2. The acute toxicity (96h-LC50) of sodium ferric EDTA towards Daphnia magna was investigated in a multispecies test system (published by Ewell et al., 1986). The 96h-LC50 was 32 mg/l (nominal; published, reliability 2).
3. The acute toxicity of EDTA, Mn(II)+EDTA and Fe(III)+EDTA towards Daphnia magna was investigated (published by Sorvari and Sillanpaa 1996, Reliability 2). The 24h-EC50 for Fe(III)+EDTA expressed as Fe(III) (= 17 mg/l) equals the value for Fe(III) when tested without EDTA (16 mg/l).
The 24h-EC50 for Fe(III)+EDTA expressed as EDTA is 89.3 mg/l. This is a lower value then the 610 mg/l for EDTA on its own. This may be due to the presence of free Fe present in the EDTA-Fe mixture that is absent in the test with EDTA on its own. The 24h-EC50 expressed as the test substance is 128.3 mg/l.
4. In addition, an acute study with DTPA-FeNaH was performed in which low toxicity to daphnids was confirmed (Kean, 2013). The 48 h EC50 value was > 82.1 mg/L (measured conc).
The key study with 48h-EC50 = 100.9 mg/l was selected because this was the most relevant study providing a concrete EC50 and was carried out according to the guidelines and under GLP. The study by Kean gave a > value only. The other two studies were either longer (96h) or shorter and had other shortcomings and gaps of information.
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