Propanenitrile, 3,3'-(dodecylimino)bis-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study under GLP without deviations.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-[(2-cyanoethyl)(dodecyl)amino]propanenitrile

EC Number:

605-014-6

Cas Number:

1555-62-0

Molecular formula:

C18H33N3

IUPAC Name:

3-[(2-cyanoethyl)(dodecyl)amino]propanenitrile

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Origin: wastewater treatment plant Hildesheim - clarifier
Bacterial density of the inoculum: 12x10^4 colony-forming units / mL
Preparation: The activated sludge was filtered through a fluted filter. The first 200 mL were discarded. The filtrate was kept under aerobic conditions to beginning of the test .

Duration of test (contact time):

35 d

Initial test substance concentration

Initial conc.:

15 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

The necessary amounts of nutrient medium and inoculum were given in each of the incubation flasks. The vessels were connected to a system of CO2-free air and ventilated for 24h.
The test and reference substance concentrations were added to the incubation vessels, filled with CO2-free double-distilled water up to 3l and connected to a system of CO2-free air.
The incubation was performed in a temperature range between 22 ± 2 ° C in a water bath.
On day 35 1 mL of conc. HCl was added to all incubation flasks.
The ventilation was continued for 24 h and on day 36, the final amount of released CO2 was determined.

Reference substance

Reference substance:

acetic acid, sodium salt

Remarks:

p.a. , >99%

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

50

Sampling time:

35 d

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

40

Sampling time:

28 d

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

25

Sampling time:

20 d

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

16

Sampling time:

13 d

% Degradation 5

Parameter:

% degradation (CO2 evolution)

Value:

2

Sampling time:

6 d

BOD5 / COD results

Results with reference substance:

83% at day 13

Any other information on results incl. tables

day	test substance [15mg/l] - test 1			test substance [15mg/l]  - test2			Control	reference substance
	mgCO2		degradation	mgCO2		degradation	mgCO2	degradation
	brutto	netto	[%]	brutto	netto	[%]		[%]
1	1.5	0	0	0	0	0	4.6	0
3	7.8	0	0	5.8	0	0	10.6	27
6	24.3	5.2	4	16 .9	0	0	19.1	51
8	38.2	12.6	10	30.9	5.3	4	25.6	66
10	47.9	16.7	14	42.9	11.7	10	31.2	74
13	58.6	21.2	17	54.8	17.4	14	37.4	83
17	75.3	28.4	23	71.9	25.0	20	46.9	93
20	84.9	31.4	26	83.3	29.8	24	53.5	93
24	103.3	41.7	34	97.3	35.7	29	61.6	100
28	119.5	50.3	41	115.9	46.7	38	69.2	100
31	131.2	56.1	46	130.9	55.8	46	75.1	100
35	142.1	58.5	48	145.1	61.5	50	83.6	100
36	147.2	58.3	48	150.9	62.0	51	88.9	100
36	148.6	58.3	48	152.6	62.3	51	90.3	100

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not ready biodegradable

Conclusions:

N,N-Di-(2-cyanoethyl)-dodecylnitril is not ready biodegradable according to OECD301B test.

Executive summary:

N,N-Di-(2-cyanoethyl)-dodecylnitril is not ready biodegradable according to OECD301B test.