2-tert-butylhydroquinone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test was performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to understand the effect of test chemical on the mortality to fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test solution was prepared by dissolving 1.8 mg, 3 mg, 4.8 mg, 7.6 mg & 12.2 mg of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 0.18 mg/L, 0.3 mg/L,0.48 mg/L, 0.76 mg/L & 1.22 mg/L, respectively

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Orange City Aquarium, Sitabuldi, Nagpur, MS (India)
- Length at study initiation (length definition, mean, range and SD): average 2.28 cm
- Weight at study initiation (mean and range, SD): average 0.115 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12-hour darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

25 mg of CaCO3

Test temperature:

23.3-25.4°C

pH:

6.24

Dissolved oxygen:

5.94

Nominal and measured concentrations:

Nominal concentration 0.18 mg/L, 0.3 mg/L,0.48 mg/L, 0.76 mg/L & 1.22 mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.3 - < 0.48 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortality and symptoms

Nominal conc. (mg/l)	Number of fish	Mortality (cumulated) after
		24h	48h	72h	96h
0 (control)	8	-	-	-	-
0.18	8	-	-	-	-
0.3	8	-	-	1	1
0.48	8	-	1	7	-
0.76	8	1	7	-	-
1.22	8	8	-	-	-

Visible Symptoms: Fishes were swimming at the bottom of bowl

 

Nominal conc. (mg/l)	Number of fish	Symptoms after
		24h	48h	72h	96h
0 (control)	8	NS8	NS8	NS8	NS8
0.18	8	D8	D8	D8	D8
0.3	8	D8	D8	D7	D7

 

 

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical to Zebra Fish Danio rerio was determine as >0.3 - <0.48 mg/L.

Executive summary:

Study was conducted to access the effect of test chemical to fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. The nominal concentrations of the test chemical ranging from 0.18 o 2.4 mg/L was taken for the study and exposed to these concentrations for 96 hours.

A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test chemical to various nominal test concentrations, LC50 was determine to be >0.3 - <0.48 mg/L. . Based on the LC50, it can be consider that the chemical can be consider to be classified in to aquatic acute category 1 as per the CLP classification criteria.

Description of key information

Study was conducted to access the effect of test chemical to fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. The nominal concentrations of the test chemical ranging from 0.18 o 2.4 mg/L was taken for the study and exposed to these concentrations for 96 hours. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test chemical to various nominal test concentrations, LC50 was determine to be >0.3 - <0.48 mg/L. . Based on the LC50, it can be consider that the chemical can be consider to be classified in to aquatic acute category 1 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.48 mg/L

Additional information

Summarized result for the toxicity of test chemical were studied and mention as below:

 

The first experimental key study was conducted to access the effect of test chemical to fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. The nominal concentrations of the test chemical ranging from 0.18 o 2.4 mg/L was taken for the study and exposed to these concentrations for 96 hours. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test chemical to various nominal test concentrations, LC50 was determine to be >0.3 - <0.48 mg/L. . Based on the LC50, it can be consider that the chemical can be consider to be classified in to aquatic acute category 1 as per the CLP classification criteria.

 

First study was supported by the second study from peer reviewed journal. Static toxicity tests were performed according to ASTM standard methods for fish. Test conducted under the static system for 48 hrs on Channel catfish. After the exposure of chemical with fish, effect on the mortality were observed. The LC50 (48 hr) value of test chemical to Ictalurus punctatus in 48 hours study on basis of effect of mortality was reported to be 0.37 mg/L. Thus based on the LC50 value, chemical consider to be toxic and classified as aquatic acute 1 as per the CLP classification criteria.

 

Similar static toxicity tests were performed according to ASTM standard methods for fish. Lepomis macrochirus exposed with chemical for 48 hrs. Fish were not fed during the experiment. In a 20-L glass jar, 15 L well water was used for the test. Mortality was measured at 1, 3, 6, 24, 48 hours. After the exposure of test chemical to Lepomis macrochirus in 48 hours study is reported to be 0.15 mg/L based on the mortality of fish. Thus chemical consider to be toxic and classified as aquatic acute 1 as per the CLP classification criteria.

 

Based on the above all studies from various sources, chemical consider to be toxic and classified in aquatic acute 1 category as per the CLP classification criteria.