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EC number: 245-629-3 | CAS number: 23386-52-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- GLP and test guideline compliant study, oxygen concentration too low at higher concentrations, good documentation, no dose verification performed
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The definitive test concentrations were obtained by transferring appropriate weights of test item directly to the test chambers.
- Controls: blank controls
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): NA
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): NA
- Evidence of undissolved material (e.g. precipitate, surface film, etc): There was some undissolved compound in each test vessel at 0-hour. All material had completely dissolved after 24 hours, although the 1000 mg/L solution was slightly cloudy.
- Test solution preparation:
The compound was allowed to stay in the water sample until it became partly dissolved at which time the partly dissolved compound solution was added to the test vessels and vigorously stirred. Fish were not added to the test solutions until the very end of the 30 minutes time limit in order to allow for more complete dissolution of the test compound. All test concentrations were based on teh total compound, i.e., not corrected for purity or undissolved material. - Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- TEST ORGANISM
- Common name: bluegill sunfish
- Source: Osage Catfisheries, Osage Beach
- Length at study initiation (length definition, mean, range and SD): 22 (+/- 1.1 ) mm
- Weight at study initiation (mean and range, SD): 0.23 (+/- 0.043) g/fish
- Feeding during test: the fish were not fed during the exposure
ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: standard commercial fish food
- Feeding frequency: daily, discontinued 48 to 96 hours prior to testing
QUARANTINE (wild caught)
- Duration: at least 14 days - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 40-48 mg/L as CaCO3
- Test temperature:
- 21 - 22°C
- pH:
- 6.7 - 7.3
- Dissolved oxygen:
- 1.1 - 8.5 mg/L
- Nominal and measured concentrations:
- nominal concentrations: 0 (control), 240, 320, 420, 560, 750 and 1000 mg CT-316-87/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 5 gallon glass vessels containing 15 L test solution
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): NA
- Biomass loading rate: 0.15 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: soft reconstituted water
Following amounts were added to deionized water:
NaHCO3: 48 mg/L
CaSO4*2H2O: 30 mg/L
MgSO4: 30 mg/L
KCl: 2 mg/L
- Metals: measured, levels below levels of concern
- Pesticides: measured, levels below levels of concern
- Alkalinity: 25 - 35 mg/L as CaCO3
- Intervals of water quality measurement: measured at 24, 48 and 96 hours
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mortality and behavioural changes
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.3
- Justification for using less concentrations than requested by guideline: NA
- Range finding study: two range finding studies were performed:
- Test concentrations:
first range finder: 1, 10, 100 mg CT-316-87/L
second range finder: 1000 mg CT-316-87/L
third range finder: 320 mg CT-316-87/L
- Results used to determine the conditions for the definitive study:
first range finder: 1, 10, 100 mg CT-316-87/L with 0/5, 0/5, 0/5 dead fish after 96 hours;
second range finder: 1000 mg CT-316-87/L with 3/5 dead fish after 48 hours
third range finder: 320 mg CT-316-87/L with 0/5 dead fish after 96 hours - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 777.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 810 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% C.I.: 710 - 960 mg test material/L
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 451.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 470 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% C.I.: 420 - 530 mg test material/L
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 451.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 470 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% C.I.: 420 - 530 mg test material/L
- Details on results:
- - Behavioural abnormalities: none reported
- Observations on body length and weight: NA
- Other biological observations: no behavioural changes at 240 mg CT-316-87/L
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: At concentrations of 320 mg/L and higher surfacing, loss of equilibrium, fish on the bottom of the test vessel, quiescence and / or laboured respiration was observed.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
After preparation of the test solutions there was undissolved compound in all (treated) test vessels. After 24 hours, this compound was dissolved in all test vessels. At 1000 mg/L the test solution was slightly cloudy. After 48 hours and for the reminder of the study, all test solutions from 240 to 1000 mg/L were slightly cloudy. The opacity increased slightly with the increase in concentration.
- Effect concentrations exceeding solubility of substance in test medium: Apparently, all test solutions were above the limit of water solubility. - Reported statistics and error estimates:
- The LC50 values for CT-316-87 were determined using the probit method.
- Sublethal observations / clinical signs:
Table 1 Nominal concentration of CT-316-87 and corresponding concentrations of CH-SSS in the test solutions (calculated for 96% purity)
Nominal concentration
[mg CT-316-87/L]
Nominal concentration
[mg a.i./L]
0 (Control)
0 (Control)
240
230.4
320
307.2
420
403.2
560
537.6
750
720
1000 960 Table 2 Temperature, dissolved oxygen and pH values measured in the test vessels at different time intervals
Nominal concentration
[mg CT-316-87 /L]
0 hours
48 hours
96 hours
Temp
[°C]
DO
[mg/L]
pH
Temp
[°C]
DO
[mg/L]
pH
Temp
[°C]
DO
[mg/L]
pH
0 (Control)
21
8.5
7.5
22
6.5
7.3
22
6.2
7.2
240
21
8.5
7.7
22
3.2
6.9
22
4.0
6.9
320
-
-
-
22
2.1
6.8
22
3.4
6.8
420 21 8.5 7.7 22 1.2 6.7 22 3.3 6.8 560 - - - 22 1.1 6.7 22 1.6 6.7 750 - - - - - - - - - 1000 21 8.5 7.6 - - - - - - Table 3 Percent mortality at the individual observation intervals
Nominal concentration
[mg CT-316-87 /L]
Cumulative Percent Mortality
24 hours
48 hours
96 hours
0 (Control)
0
0
0
240
0
0
0
320
0
0
0
420
0
20
20
560
10
90
100
750
20
100
100
1000 90 100 100 - Validity criteria fulfilled:
- yes
- Remarks:
- the control validity criteria were fulfilled, the DO concentration criteria not at higher concentrations
- Conclusions:
- 96-hour LC50 (Lepomis macrochirus)=451.2 mga.i./L
- Executive summary:
The acute toxicity of CT-316 -87 - which contains 96% a.i. - to bluegill sunfish, Lepomis macrochirus, was determined in a static test. The test was performed according to OECD 203. Deviations to the guideline were significant oxygen reduction in the higher test groups which might have resulted in mortality. The test solutions were not aerated. No dose-verification analysis was performed. The test was performed with a control and nominal 240, 320, 420, 560, 750 and 1000 mg CT-316 -87/L corresponding to 230.4, 307.2, 403,2, 537,6, 720 and 960 mg a.i./L. During the study, the temperature of the test solutions ranged from 21 to 22°C. During the study the oxygen concentration declined with increasing test substance concentration. The lowest dissolved oxygen concentration measured was 1.1 mg/L. After 96 hours of exposure, 0, 0, 0, 2, 9, 10 and 10 out of 10 fish had died in the control, and 240, 320, 420, 560, 750 and 1000 mg CT-316 -87/L treatment groups. The 96 h LC50 was determined to be 470 mg CT-316 -87/L corresponding to 451.2 mg a.i./L. The concentrations were based on nominal concentrations.
The results of this study need to be interpreted with care since the dissolved oxygen concentrations might have had a significant impact on the mortality observed during the test. However, since the potential mortality caused by oxygen reduction is considered to increase the mortality and hence represents the worst case scenario the results are considered to be reliable with restrictions for the risk assessment.
Reference
Description of key information
LC50 bluegill sunfish: 451.2 mga.i./L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 451.2 mg/L
Additional information
The study from Cytec (Bowman 1987) was performed under GLP was scored as Klimisch 2 study. The acute toxicity of CT-316 -87- which contains 96% a.i. - to bluegill sunfish, Lepomis macrochirus, was determined in a static test. The test was performed according to OECD 203. Deviations to the guideline were significant oxygen reduction in the higher test groups which might have resulted in mortality. The test solutions were not aerated. No dose-verification analysis was performed. The test was performed with a control and nominal 240, 320, 420, 560, 750 and 1000 mg CT-316 -87/L corresponding to 230.4, 307.2, 403,2, 537,6, 720 and 960 mg a.i./L. During the study, the temperature of the test solutions ranged from 21 to 22°C. During the study the oxygen concentration declined with increasing test substance concentration. The lowest dissolved oxygen concentration measured was 1.1 mg/L. After 96 hours of exposure, 0, 0, 0, 2, 9, 10 and 10 out of 10 fish had died in the control, and 240, 320, 420, 560, 750 and 1000 mg CT-316 -87/L treatment groups. The 96 h LC50 was determined to be 470 mg CT-316 -87/L corresponding to 451.2 mg a.i./L.The concentrations were based on nominal concentrations.
The results of this study need to be interpreted with care since the dissolved oxygen concentrations might have had a significant impact on the mortality observed during the test. However, since the potential mortality caused by oxygen reduction is considered to increase the mortality and hence represents the worst case scenario the results are considered to be reliable with restrictions for the risk assessment.
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