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EC number: 217-316-1 | CAS number: 1809-19-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 June to August, 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dibutyl phosphonate
- EC Number:
- 217-316-1
- EC Name:
- Dibutyl phosphonate
- Cas Number:
- 1809-19-4
- Molecular formula:
- C8H19O3P
- IUPAC Name:
- dibutyl phosphonate
- Test material form:
- liquid
Constituent 1
Method
- Target gene:
- No data
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- Salmonella typhimurium
- Species / strain / cell type:
- S. typhimurium TA 1538
- Details on mammalian cell type (if applicable):
- Salmonella typhimurium
- Species / strain / cell type:
- Saccharomyces cerevisiae
- Details on mammalian cell type (if applicable):
- Strain: D4
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- 0.001, 0.01, 0.1, 1.0 and 5.0 µL
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: deionized water or DMSO
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: methylnitrosoguanidine
- Remarks:
- without S9
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- other:
- Remarks:
- without S9
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: quinacrine mustard
- Remarks:
- without S9
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2-Antramine
- Remarks:
- with S9
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-acetylaminofluorene
- Remarks:
- with S9
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 8-Aminoquinoline
- Remarks:
- with S9
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar
Approximately 10^6 cells from overnight culture of each indicator strain were added to separate test tubes containing 2.0 mL of molten agar supplemented with biotin and trace of histidine. For activation test, at least four dose levels of test compound were added to the contents of the appropriate tubes and poured over the surface of selection of four different concentrations of the test chemical were added to the contents of the appropriate tubes with cells.
Just prior to pouring, an aliquot of reaction mixture (0.5 mL containing the 9000 x g liver homogenate) was added to each of the activation overlay tubes, which were then mixed, and the contents poured over the sacrifice of a minimal agar plate and allowed to solidify. The plates were incubated for 48 hours at 37 °C, and scored for the number of colonies growing on each plate. The concentrations of all chemicals are given in the result section. Positive and solvent controls using both directly active positive chemicals and those that require metabolic activation were run with each assay.
STUDY DESIGN
The compound was tested over a series of concentrations such that chemically-induced physiological effects at the high dose level. The low dose in all cases was below a concentration that demonstrated any toxic effect. The dose range employed for the evaluation of this compound was from 0.001 µL to 5 µL per plate. The compound was toxic to all the strains at 5 µL per plate. - Statistics:
- The number of colonies on each plate were counted and recorded on printed form. These raw data analyzed in a computer program and reported on a printout. The results are presented as revertant per plate for each indicator strain employed in the assay. The positive and the solvent controls are provided as reference points. Other relevant data are provided on the computer printout.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- Saccharomyces cerevisiae
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Toxicity test:
- The dose range employed for the evaluation of this of this compound was from 0.001 µL to 5 µL per plate. The compound was toxic to all the strains at 5µL per plate.
Nonactivation test results:
- The results of the tests conducted on the compound in the absence of a metabolic system were all negative.
Activation test results:
- The results of the tests conducted on the compound in the presence of the rat liver activation system were all negative. - Remarks on result:
- other: all strains/cell types tested
Applicant's summary and conclusion
- Conclusions:
- Negative with and without metabolic activation
- Executive summary:
The potential for the substance to induce an increase in reverse mutatitions in bacterial cells was evaluated in an AMES test, equivalent to the OECD Guideline 471. The objective of this test was to evaluate the compound for genetic activity in microbial assays of Salmonella typhimurium TA 1535, TA 1537, TA1538, TA 98 and TA 100, as well as Saccharomyces cerevisiae strain D4, with and without the addition of mammalian metabolic activation preparation, at test concentrations of 0.001, 0.01, 0.1, 1.0 and 5.0 µL.
The compound was toxic to all the strains at 5 µL per plate. The test item did not demonstrate increased mutagenic activity in any of the assays conducted in this evaluation and was considered not mutagenic under these test conditions.
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